Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Faithful Makita-Chingombe, Anthony T. Podany, Timothy Mykris, Farai Muzambi, Richard W. Browne, Andrew J. Ocque, Robin DiFrancesco, Lee C. Winchester, Courtney V. Fletcher, Tinashe Mudzviti, Charles C. Maponga, Gene D. Morse

Research output: Contribution to journalArticlepeer-review

Abstract

An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

Original languageEnglish (US)
Article numbera1264
JournalAfrican Journal of Laboratory Medicine
Volume10
Issue number1
DOIs
StatePublished - 2021

Keywords

  • Cross-validation
  • HIV
  • High-performance liquid chromatography
  • Root-cause analysis

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Clinical Biochemistry
  • Medical Laboratory Technology

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