Crystallization and initial X-ray analysis of alkaline xylanase

Ning Lu; Hideaki Moriyama; Satoshi Nakamura; Takao Sato; Nobuo Tanaka

doi:10.1107/S0907444900000913

Crystallization and initial X-ray analysis of alkaline xylanase

Ning Lu, Hideaki Moriyama, Satoshi Nakamura, Takao Sato, Nobuo Tanaka

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P4₁ or P4₃ and unit-cell parameters a = b = 115.7, c = 46.0 Å. Assuming two molecules per asymmetric unit, V(m) = 2.31 Å³ Da^-1. The crystals diffract X-rays to 2.6 Å resolution at 100 K.

Original language	English (US)
Pages (from-to)	464-465
Number of pages	2
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	56
Issue number	4
DOIs	https://doi.org/10.1107/S0907444900000913
State	Published - 2000
Externally published	Yes

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Structural Biology

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title = "Crystallization and initial X-ray analysis of alkaline xylanase",

abstract = "Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P41 or P43 and unit-cell parameters a = b = 115.7, c = 46.0 {\AA}. Assuming two molecules per asymmetric unit, V(m) = 2.31 {\AA}3 Da-1. The crystals diffract X-rays to 2.6 {\AA} resolution at 100 K.",

author = "Ning Lu and Hideaki Moriyama and Satoshi Nakamura and Takao Sato and Nobuo Tanaka",

year = "2000",

doi = "10.1107/S0907444900000913",

language = "English (US)",

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T1 - Crystallization and initial X-ray analysis of alkaline xylanase

AU - Lu, Ning

AU - Moriyama, Hideaki

AU - Nakamura, Satoshi

AU - Sato, Takao

AU - Tanaka, Nobuo

PY - 2000

Y1 - 2000

N2 - Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P41 or P43 and unit-cell parameters a = b = 115.7, c = 46.0 Å. Assuming two molecules per asymmetric unit, V(m) = 2.31 Å3 Da-1. The crystals diffract X-rays to 2.6 Å resolution at 100 K.

AB - Crystals of alkaline xylanase (xylanase J) from alkaliphilic Bacillus sp. 41M-1 were grown by decreasing the temperature of the protein solution. Initial X-ray analysis of the crystal showed a tetragonal system with space group P41 or P43 and unit-cell parameters a = b = 115.7, c = 46.0 Å. Assuming two molecules per asymmetric unit, V(m) = 2.31 Å3 Da-1. The crystals diffract X-rays to 2.6 Å resolution at 100 K.

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JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 4

ER -

Crystallization and initial X-ray analysis of alkaline xylanase

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