TY - JOUR
T1 - Crystallization and preliminary crystallographic analysis of the complex of the second and third regulatory subunits of human Pol δ
AU - Baranovskiy, Andrey G.
AU - Babayeva, Nigar D.
AU - Pavlov, Youri I.
AU - Tahirov, Tahir H.
PY - 2008
Y1 - 2008
N2 - Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His-tagged p50 subunit (p50) and a p50-interacting domain of the p66 subunit (p66N) was crystallized. The crystal was in the form of a prism with a rhombic cross-section and belonged to space group P21. The crystal had unit-cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50-p66N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.
AB - Human DNA polymerase δ (Pol δ) consists of four subunits: p125, p50, p66 and p12. A heterodimer containing a His-tagged p50 subunit (p50) and a p50-interacting domain of the p66 subunit (p66N) was crystallized. The crystal was in the form of a prism with a rhombic cross-section and belonged to space group P21. The crystal had unit-cell parameters a = 95.13, b = 248.54, c = 103.46 Å, β = 106.94° and diffracted to a resolution of 3 Å. Four molecules of p50-p66N in an asymmetric unit corresponded to a crystal solvent content of 72.2%.
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U2 - 10.1107/S1744309108025086
DO - 10.1107/S1744309108025086
M3 - Article
C2 - 18765914
AN - SCOPUS:51149118870
SN - 1744-3091
VL - 64
SP - 822
EP - 824
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 9
ER -