Abstract
The nickel-responsive repressor from Pyrococcus horikoshii OT3 (PhNikR) has been crystallized in the apo form (PhNikR-apo) and two nickel-bound forms (PhNikR-Ni-1 and PhNikR-Ni-2). The PhNikR-apo crystals belong to space group P21, with unit-cell parameters a = 75.78, b = 54.32, c = 77.28 Å, β = 116.07°, and diffract to 2.2 Å. The PhNikR-Ni-1 crystals belong to space group P41212, with unit-cell parameters a = b = 99.89, c = 97.98 Å, and diffract to 3.0 Å and the PhNikR-Ni-2 crystals belong to space group P3121 or P3221, with unit-cell parameters a = b = 109.95, c = 79.0 Å, and diffract to 2.1 Å. The crystals obtained were suitable for detailed structural studies.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 43-45 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 61 |
| Issue number | 1 |
| DOIs | |
| State | Published - Dec 1 2005 |
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics
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Kitao, T., Kuroishi, C., & Tahirov, T. H. (2005). Crystallization and preliminary crystallographic analysis of the nickel-responsive regulator NikR from Pyrococcus horikoshii. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 61(1), 43-45. https://doi.org/10.1107/S1744309104025473