Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

T. H. Tahirov; T. Inoue-Bungo; M. Sasaki; M. Shiina; K. Kimura; K. Sato; T. Kumasaka; M. Yamamoto; N. Kamiya; K. Ogata

doi:10.1107/S0907444901003900

Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

T. H. Tahirov, T. Inoue-Bungo, M. Sasaki, M. Shiina, K. Kimura, K. Sato, T. Kumasaka, M. Yamamoto, N. Kamiya, K. Ogata

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C222₁, P2₁2₁2 and P2₁2₁2₁, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 Å, while that of the CBFα-DNA crystal was 2.65 Å. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.

Original language	English (US)
Pages (from-to)	850-853
Number of pages	4
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	57
Issue number	6
DOIs	https://doi.org/10.1107/S0907444901003900
State	Published - 2001
Externally published	Yes

ASJC Scopus subject areas

Structural Biology

Access to Document

10.1107/S0907444901003900

Cite this

Tahirov, T. H., Inoue-Bungo, T., Sasaki, M., Shiina, M., Kimura, K., Sato, K., Kumasaka, T., Yamamoto, M., Kamiya, N., & Ogata, K. (2001). Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region. Acta Crystallographica Section D: Biological Crystallography, 57(6), 850-853. https://doi.org/10.1107/S0907444901003900

Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region. / Tahirov, T. H.; Inoue-Bungo, T.; Sasaki, M. et al.
In: Acta Crystallographica Section D: Biological Crystallography, Vol. 57, No. 6, 2001, p. 850-853.

Research output: Contribution to journal › Article › peer-review

Tahirov, TH, Inoue-Bungo, T, Sasaki, M, Shiina, M, Kimura, K, Sato, K, Kumasaka, T, Yamamoto, M, Kamiya, N & Ogata, K 2001, 'Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region', Acta Crystallographica Section D: Biological Crystallography, vol. 57, no. 6, pp. 850-853. https://doi.org/10.1107/S0907444901003900

Tahirov TH, Inoue-Bungo T, Sasaki M, Shiina M, Kimura K, Sato K et al. Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region. Acta Crystallographica Section D: Biological Crystallography. 2001;57(6):850-853. doi: 10.1107/S0907444901003900

@article{2b92de590f0c4d9abb9c6531f60f6d00,

title = "Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region",

abstract = "Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 {\AA}, while that of the CBFα-DNA crystal was 2.65 {\AA}. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.",

author = "Tahirov, {T. H.} and T. Inoue-Bungo and M. Sasaki and M. Shiina and K. Kimura and K. Sato and T. Kumasaka and M. Yamamoto and N. Kamiya and K. Ogata",

year = "2001",

doi = "10.1107/S0907444901003900",

language = "English (US)",

volume = "57",

pages = "850--853",

journal = "Acta Crystallographica Section D: Biological Crystallography",

issn = "0907-4449",

publisher = "International Union of Crystallography",

number = "6",

}

TY - JOUR

T1 - Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

AU - Tahirov, T. H.

AU - Inoue-Bungo, T.

AU - Sasaki, M.

AU - Shiina, M.

AU - Kimura, K.

AU - Sato, K.

AU - Kumasaka, T.

AU - Yamamoto, M.

AU - Kamiya, N.

AU - Ogata, K.

PY - 2001

Y1 - 2001

N2 - Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 Å, while that of the CBFα-DNA crystal was 2.65 Å. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.

AB - Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 Å, while that of the CBFα-DNA crystal was 2.65 Å. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.

UR - http://www.scopus.com/inward/record.url?scp=14344284548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=14344284548&partnerID=8YFLogxK

U2 - 10.1107/S0907444901003900

DO - 10.1107/S0907444901003900

M3 - Article

C2 - 11375505

AN - SCOPUS:14344284548

SN - 0907-4449

VL - 57

SP - 850

EP - 853

JO - Acta Crystallographica Section D: Biological Crystallography

JF - Acta Crystallographica Section D: Biological Crystallography

IS - 6

ER -

Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this