Crystallization and preliminary X-ray analyses of quaternary, ternary and binary protein - DNA complexes with involvement of AML1/Runx-1/CBFα Runt domain, CBFβ and the C/EBPβ bZip region

T. H. Tahirov, T. Inoue-Bungo, M. Sasaki, M. Shiina, K. Kimura, K. Sato, T. Kumasaka, M. Yamamoto, N. Kamiya, K. Ogata

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Three types of protein - DNA complexes, AML1/Runx-1/ CBFα(Runt) - CBFβ - C/EBPβ(bZip) - DNA (CBFα-β-C/EBPβ-DNA), AML1/Runx-1/CBFα(Runt) - C/EBPβ(bZip) - DNA (CBFα-C/EBPβ-DNA) and AML1/Runx-1/CBFα(Runt) - DNA (CBFα-DNA), were crystallized. The crystals were all orthorhombic and belonged to space groups C2221, P21212 and P212121, respectively. The resolutions of CBFα-β-C/EBPβ-DNA and CBFα-C/EBPβ-DNA crystals were both 3 Å, while that of the CBFα-DNA crystal was 2.65 Å. Complete data sets were collected for all of the native crystals, along with MAD and MIR data sets for CBFα-β-C/EBPβ-DNA. The heavy-atom site was determined using MAD data for a gold derivative of CBFα-β-C/EBPβ-DNA.

Original languageEnglish (US)
Pages (from-to)850-853
Number of pages4
JournalActa Crystallographica Section D: Biological Crystallography
Volume57
Issue number6
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Structural Biology

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