Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

Tahir H. Tahirov, Hisayuki Morii, Hatsuho Uedaira, Akinori Sarai, Kazuhiro Ogata

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P212121. The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 Å. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.371.45 Å resolution.

Original languageEnglish (US)
Pages (from-to)1345-1347
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number7
DOIs
StatePublished - Jul 1 1999

ASJC Scopus subject areas

  • Structural Biology

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