Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

Tahir H. Tahirov; Hisayuki Morii; Hatsuho Uedaira; Akinori Sarai; Kazuhiro Ogata

doi:10.1107/S0907444999005041

Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

Tahir H. Tahirov, Hisayuki Morii, Hatsuho Uedaira, Akinori Sarai, Kazuhiro Ogata

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Abstract

The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P2₁2₁2₁. The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 Å. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.371.45 Å resolution.

Original language	English (US)
Pages (from-to)	1345-1347
Number of pages	3
Journal	Acta Crystallographica Section D: Biological Crystallography
Volume	55
Issue number	7
DOIs	https://doi.org/10.1107/S0907444999005041
State	Published - Jul 1999
Externally published	Yes

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Structural Biology

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title = "Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain",

abstract = "The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P212121. The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 {\AA}. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.371.45 {\AA} resolution.",

author = "Tahirov, {Tahir H.} and Hisayuki Morii and Hatsuho Uedaira and Akinori Sarai and Kazuhiro Ogata",

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T1 - Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

AU - Tahirov, Tahir H.

AU - Morii, Hisayuki

AU - Uedaira, Hatsuho

AU - Sarai, Akinori

AU - Ogata, Kazuhiro

PY - 1999/7

Y1 - 1999/7

N2 - The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P212121. The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 Å. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.371.45 Å resolution.

AB - The R2 subdomain of the mouse c-Myb DNA-binding domain and its V103L mutant have been crystallized by the vapour-diffusion method using highly concentrated sodium citrate at pH 6.8 as a precipitant. Using ammonium sulfate as precipitant in MES buffer only produced crystals for the mutant R2. All crystals are isomorphous and belong to space group P212121. The unit-cell dimensions for wild-type R2 crystals grown from sodium citrate precipitant are a = 28.83, b = 40.18, c = 49.23 Å. Crystals contain one R2 molecule per asymmetric unit. They are stable during 3 d exposure to X-rays and diffract to 1.371.45 Å resolution.

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Crystallization and preliminary X-ray analysis of wild-type and V103L mutant Myb R2 DNA-binding domain

Abstract

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