Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Ko Onodera; Hideaki Moriyama; Akio Takenaka; Nobuo Tanaka; Nobuko Akutsu; Masahito Muro; Tairo Oshima; Katsumi Imada; Mamoru Sato; Yukiteru Katsube

doi:10.1093/oxfordjournals.jbchem.a123328

Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Ko Onodera, Hideaki Moriyama, Akio Takenaka, Nobuo Tanaka, Nobuko Akutsu, Masahito Muro, Tairo Oshima, Katsumi Imada, Mamoru Sato, Yukiteru Katsube

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3₁21 or P3₂21, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

Original language	English (US)
Pages (from-to)	1-2
Number of pages	2
Journal	Journal of Biochemistry
Volume	109
Issue number	1
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a123328
State	Published - Jan 1991
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

Access to Document

10.1093/oxfordjournals.jbchem.a123328

Cite this

@article{e33bfb96952a4c0f90c53a123251837d,

title = "Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase",

abstract = "A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 {\AA} and c = 158.3 {\AA}, which is isomorphous with that of the native enzyme from T. thermophilus.",

author = "Ko Onodera and Hideaki Moriyama and Akio Takenaka and Nobuo Tanaka and Nobuko Akutsu and Masahito Muro and Tairo Oshima and Katsumi Imada and Mamoru Sato and Yukiteru Katsube",

year = "1991",

month = jan,

doi = "10.1093/oxfordjournals.jbchem.a123328",

language = "English (US)",

volume = "109",

pages = "1--2",

journal = "Journal of Biochemistry",

issn = "0021-924X",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

AU - Onodera, Ko

AU - Moriyama, Hideaki

AU - Takenaka, Akio

AU - Tanaka, Nobuo

AU - Akutsu, Nobuko

AU - Muro, Masahito

AU - Oshima, Tairo

AU - Imada, Katsumi

AU - Sato, Mamoru

AU - Katsube, Yukiteru

PY - 1991/1

Y1 - 1991/1

N2 - A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

AB - A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

UR - http://www.scopus.com/inward/record.url?scp=0026008531&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026008531&partnerID=8YFLogxK

U2 - 10.1093/oxfordjournals.jbchem.a123328

DO - 10.1093/oxfordjournals.jbchem.a123328

M3 - Article

C2 - 1901851

AN - SCOPUS:0026008531

VL - 109

SP - 1

EP - 2

JO - Journal of Biochemistry

JF - Journal of Biochemistry

SN - 0021-924X

IS - 1

ER -

Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this