Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Ko Onodera; Hideaki Moriyama; Akio Takenaka; Nobuo Tanaka; Nobuko Akutsu; Masahito Muro; Tairo Oshima; Katsumi Imada; Mamoru Sato; Yukiteru Katsube

doi:10.1093/oxfordjournals.jbchem.a123328

Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

Ko Onodera, Hideaki Moriyama, Akio Takenaka, Nobuo Tanaka, Nobuko Akutsu, Masahito Muro, Tairo Oshima, Katsumi Imada, Mamoru Sato, Yukiteru Katsube

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3₁21 or P3₂21, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

Original language	English (US)
Pages (from-to)	1-2
Number of pages	2
Journal	Journal of Biochemistry
Volume	109
Issue number	1
DOIs	https://doi.org/10.1093/oxfordjournals.jbchem.a123328
State	Published - Jan 1991
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase. / Onodera, Ko; Moriyama, Hideaki; Takenaka, Akio; Tanaka, Nobuo; Akutsu, Nobuko; Muro, Masahito; Oshima, Tairo; Imada, Katsumi; Sato, Mamoru; Katsube, Yukiteru.

In: Journal of Biochemistry, Vol. 109, No. 1, 01.1991, p. 1-2.

Research output: Contribution to journal › Article › peer-review

Onodera, Ko ; Moriyama, Hideaki ; Takenaka, Akio ; Tanaka, Nobuo ; Akutsu, Nobuko ; Muro, Masahito ; Oshima, Tairo ; Imada, Katsumi ; Sato, Mamoru ; Katsube, Yukiteru. / Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase. In: Journal of Biochemistry. 1991 ; Vol. 109, No. 1. pp. 1-2.

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abstract = "A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 {\AA} and c = 158.3 {\AA}, which is isomorphous with that of the native enzyme from T. thermophilus.",

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AU - Takenaka, Akio

AU - Tanaka, Nobuo

AU - Akutsu, Nobuko

AU - Muro, Masahito

AU - Oshima, Tairo

AU - Imada, Katsumi

AU - Sato, Mamoru

AU - Katsube, Yukiteru

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AB - A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.

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Crystallization and preliminary x-ray studies of a Bacillus subtilis and Thermuts thermophilus HB8 chimeric 3-isopropylmalate dehy drogenase

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