Abstract
A chimeric gene was constructed by fusing the Bacillus subtilis and Thermus thermophilus genes coding for 3-isopropylmalate dehydrogenase, and expressed in Escherichia coli. The chimeric enzyme was crystallized in a size suitable for X-ray structure analysis. The crystal has a space group of P3121 or P3221, a=b=77.1 Å and c = 158.3 Å, which is isomorphous with that of the native enzyme from T. thermophilus.
Original language | English (US) |
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Pages (from-to) | 1-2 |
Number of pages | 2 |
Journal | Journal of Biochemistry |
Volume | 109 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1991 |
Externally published | Yes |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology