Crystallization and preliminary X-ray studies on the hyperstable 3-isopropylmalate dehydrogenase from the thermoacidophilic archaeon Sulfolobus sp. strain 7

Toshiharu Suzuki, Hideaki Moriyama, Raita Hirose, Masahiro Sakurai, Nobuo Tanaka, Tairo Oshima

Research output: Contribution to journalArticle

Abstract

3-Isopropylmalate dehydrogenase from the thermoacidophilic archaeon, Sulfolobus sp. strain 7, has been crystallized by the vapor-diffusion method. The crystals were grown from a solution containing ammonium sulfate, 2-methyl-2,4-pentanediol and magnesium chloride. The crystallization requires 2-methyl-2,4-pentanediol to avoid twinning of the crystals. The crystal belongs to the orthorhombic system with the space group P2221 and unit-cell dimensions a = 67.9,b = 93.3 and c = 134.1 Å.

Original languageEnglish (US)
Pages (from-to)444-445
Number of pages2
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number3
DOIs
StatePublished - May 1 1998

ASJC Scopus subject areas

  • Structural Biology

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