TY - JOUR
T1 - Culture Methods for the Detection of Minimal Tumor Contamination of Hematopoietic Harvests
T2 - A Review
AU - Sharp, J. Graham
AU - Mann, Sally L.
AU - Murphy, Barbara
AU - Weekes, Colin
PY - 1995/6
Y1 - 1995/6
N2 - The evaluation of minimal residual disease in patients and hematopoietic cell grafts is of considerable importance for staging disease, determining the response to treatment, and monitoring the efficiency of ex vivo purging or positive selection procedures. The most widely used techniques are immunocytochemical staining and the polymerase chain reaction; however, these assays do not measure the viability or clonogenic capacity of the detected cells. For this purpose, a culture technique must be used. This paper reviews the status, advantages, and limitations of this approach and the detection of tumor cells in bone marrow and peripheral blood.
AB - The evaluation of minimal residual disease in patients and hematopoietic cell grafts is of considerable importance for staging disease, determining the response to treatment, and monitoring the efficiency of ex vivo purging or positive selection procedures. The most widely used techniques are immunocytochemical staining and the polymerase chain reaction; however, these assays do not measure the viability or clonogenic capacity of the detected cells. For this purpose, a culture technique must be used. This paper reviews the status, advantages, and limitations of this approach and the detection of tumor cells in bone marrow and peripheral blood.
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U2 - 10.1089/scd.1.1995.4.141
DO - 10.1089/scd.1.1995.4.141
M3 - Article
C2 - 7551913
AN - SCOPUS:0028865898
SN - 1525-8165
VL - 4
SP - 141
EP - 148
JO - Journal of Hematotherapy and Stem Cell Research
JF - Journal of Hematotherapy and Stem Cell Research
IS - 3
ER -