Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal

Hisatoshi Sugiura, Xiangde Liu, Fenghai Duan, Shin Kawasaki, Shinsaku Togo, Koichiro Kamio, Qi Wang Xing, Lijun Mao, Youngsoo Ahn, Ronald F. Ertl, Tom W. Bargar, Abdo Berro, Thomas B. Casale, Stephen I. Rennard

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Asthmatic airway remodeling is characterized by goblet cell hyperplasia, angiogenesis, smooth muscle hypertrophy, and subepithelial fibrosis. This study evaluated whether acquired changes in fibroblast phenotype could contribute to this remodeling. Airway and parenchymal fibroblasts from control or chronically ovalbumin (OVA)-sensitized and challenged "asthmatic" mice were assessed for several functions related to repair and remodeling ± exogenous transforming growth factor (TGF)-β. All OVA-challenged mouse fibroblasts demonstrated augmented gel contraction (P < 0.05) and Chemotaxis (P < 0.05); increased TGF-β1 (P < 0.05), fibronectin (P < 0.05), and vascular endothelial growth factor (P < 0.05) release; and expressed more α-smooth muscle actin (P < 0.05). TGF- aβ1 stimulated both control and asthmatic fibroblasts, which retained all differences from control fibroblasts for all features(P < 0.05, all comparisons). Parenchymal fibroblasts proliferated more rapidly (P < 0.05), while airway fibroblasts proliferated similarly compared with control fibroblasts (P = 0.25). Thus, in this animal model, OVA-challenged mouse fibroblasts acquire a distinct phenotype that differs from control fibroblasts. The augmented profibrotic activity and mediator release of asthmatic fibroblasts could contribute to airway remodeling in asthma.

Original languageEnglish (US)
Pages (from-to)424-430
Number of pages7
JournalAmerican journal of respiratory cell and molecular biology
Volume37
Issue number4
DOIs
StatePublished - Oct 2007

Keywords

  • Fibroblast
  • Mouse model
  • Phenotype
  • Remodeling

ASJC Scopus subject areas

  • Molecular Biology
  • Pulmonary and Respiratory Medicine
  • Clinical Biochemistry
  • Cell Biology

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    Sugiura, H., Liu, X., Duan, F., Kawasaki, S., Togo, S., Kamio, K., Xing, Q. W., Mao, L., Ahn, Y., Ertl, R. F., Bargar, T. W., Berro, A., Casale, T. B., & Rennard, S. I. (2007). Cultured lung fibroblasts from ovalbumin-challenged "asthmatic" mice differ functionally from normal. American journal of respiratory cell and molecular biology, 37(4), 424-430. https://doi.org/10.1165/rcmb.2007-0089OC