Current perspectives and recommendations for the development of mass spectrometry methods for the determination of allergens in foods

Phil E. Johnson, Sabine Baumgartner, Thomas Aldick, Conrad Bessant, Valeria Giosafatto, Julia Heick, Gianfranco Mamone, Gavin O'Connor, Roland Poms, Bert Popping, Andreas Reuter, Franz Ulberth, Andrew Watson, Linda Monaci, E. N.Clare Mills

Research output: Contribution to journalReview article

84 Scopus citations

Abstract

Allergen detection and quantification is an essential part of allergen management as practiced by food manufacturers. Recently, protein MS methods (in particular, multiple reaction monitoring experiments) have begun to be adopted by the allergen detection community to provide an alternative technique to ELISA and PCR methods. MS analysis of proteins in foods provides additional challenges to the analyst, both in terms of experimental design and methodology: (1) choice of analyte, including multiplexing to simultaneously detect several biologically relevant molecules able to trigger allergic reactions; (2) choice of processing stable peptide markers for different target analytes that should be placed in publicly available databases; (3) markers allowing quantification (e.g., through standard addition or isotopically labeled peptide standards); (4) optimization of protease digestion protocols to ensure reproducible and robust method development; and (5) effective validation of methods and harmonization of results through the use of naturally incurred reference materials spanning several types of food matrix.

Original languageEnglish (US)
Pages (from-to)1026-1033
Number of pages8
JournalJournal of AOAC International
Volume94
Issue number4
StatePublished - Jul 2011

ASJC Scopus subject areas

  • Analytical Chemistry
  • Food Science
  • Environmental Chemistry
  • Agronomy and Crop Science
  • Pharmacology

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    Johnson, P. E., Baumgartner, S., Aldick, T., Bessant, C., Giosafatto, V., Heick, J., Mamone, G., O'Connor, G., Poms, R., Popping, B., Reuter, A., Ulberth, F., Watson, A., Monaci, L., & Mills, E. N. C. (2011). Current perspectives and recommendations for the development of mass spectrometry methods for the determination of allergens in foods. Journal of AOAC International, 94(4), 1026-1033.