Cyclooxygenase inhibition attenuates cholecystokinin-induced gastroprotection

David W. Mercer, Gregory S. Smith, Thomas A. Miller

Research output: Contribution to journalArticlepeer-review

10 Scopus citations


Cholecystokinin prevents gastric injury by an unknown mechanism. This study was conducted in conscious, fasted female rats in order to assess the role of endogenous prostaglandins as a potential protective mechanism for cholecystokinin-induced gastroprotection. Intravenous administration of cholecystokinin (0,05-5 nmol/kg) dose-dependently reduced macroscopic injury to the glandular portion of the stomach caused by 1 ml of orally administered acidified ethanol (150 mM hydrochloric acid-50% ethanol), an effect corroborated by histologic analysis. In time course studies, this protective action occurred as early as 10 min following cholecystokinin injection (5 nmol/kg intravenously), but was absent at 1 hr. Cyclooxygenase inhibition with either indomethacin (5 mg/kg intraperitoneally) or aspirin (100 mg/kg intraperitoneally) resulted in a partial reversal in cholecystokinin-induced gastroprotection, effects that were similar in magnitude. However, while indomethacin reduced gastric mucosal prostaglandin synthesis (enzyme-linked immunoassay) by 60%, aspirin almost totally abolished prostaglandin synthesis (95% reduction). Cholecystokinin (5 nmol/kg intravenously) did not significantly enhance gastric mucosal prostaglandin synthesis in the absence of cyclooxygenase inhibition. These data indicate that cholecystokinin requires the presence of endogenous prostaglandins in order to fully exert its gastroprotective actions. However, release of endogenous prostaglandins does not entirely explain the protective response, and additional factors likely participate in this action.

Original languageEnglish (US)
Pages (from-to)468-475
Number of pages8
JournalDigestive Diseases and Sciences
Issue number3
StatePublished - 1998
Externally publishedYes


  • Aspirin
  • Cholecystokinin
  • Gastric injury
  • Indomethacin
  • Prostaglandins

ASJC Scopus subject areas

  • Physiology
  • Gastroenterology


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