Cyclophosphoramidate ion as mass defect marker for efficient detection of protein serine phosphorylation

Yu Shi, Bekim Bajrami, Martha Morton, Xudong Yao

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


A novel method is reported to modify the phosphate groups on phosphoserine peptides to the corresponding phosphoramidates, using 2-aminobenzylamine. Upon collision-induced dissociation, the modified peptides release the positively charged phosphoramidate that via gas-phase intramolecular elimination forms a cyclophosphoramidate (CyPAA) ion, the protonated form of 1,4-dihydro-2-hydroxy- 2-oxobenzo[3,1,2]oxazaphosphorine. The positive nature of the ion eliminates the need for real-time instrument polarity switching and greatly increases the versatility of commonly used mass spectrometers for phosphopeptide analysis. This ion has sufficient mass defect, due to containing a phosphorus atom and a high content of oxygen atoms, which makes mass spectrometers of medium mass resolution and accuracy adequate for separating the ion from isobaric interfering ions. The specificity of the CyPAA ion for detecting phosphoserine peptides in complex peptide mixtures is comparable to the specificity of the phosphotyrosine immonium ion for phosphotyrosine peptides, allowing the efficient data complexity reduction for fast and focused analysis of phosphoserine-containing peptides.

Original languageEnglish (US)
Pages (from-to)7614-7623
Number of pages10
JournalAnalytical chemistry
Issue number19
StatePublished - Oct 1 2008
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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