Decoding cell lineage from acquired mutations using arbitrary deep sequencing

Cheryl A. Carlson, Arnold Kas, Robert Kirkwood, Laura E. Hays, Bradley D. Preston, Stephen J. Salipante, Marshall S. Horwitz

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Because mutations are inevitable, the genome of each cell in a multicellular organism becomes unique and therefore encodes a record of its ancestry. Here we coupled arbitrary single primer PCR with next-generation DNA sequencing to catalog mutations and deconvolve the phylogeny of cultured mouse cells. This study helps pave the way toward construction of retrospective cell-fate maps based on mutations accumulating in genomes of somatic cells.

Original languageEnglish (US)
Pages (from-to)78-80
Number of pages3
JournalNature Methods
Volume9
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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