@article{1f48d9c7c24444cbb931bf571ab46d90,
title = "Dedicated Industrial Oilseed Crops as Metabolic Engineering Platforms for Sustainable Industrial Feedstock Production",
abstract = "Feedstocks for industrial applications ranging from polymers to lubricants are largely derived from petroleum, a non-renewable resource. Vegetable oils with fatty acid structures and storage forms tailored for specific industrial uses offer renewable and potentially sustainable sources of petrochemical-type functionalities. A wide array of industrial vegetable oils can be generated through biotechnology, but will likely require non-commodity oilseed platforms dedicated to specialty oil production for commercial acceptance. Here we show the feasibility of three Brassicaceae oilseeds crambe, camelina, and carinata, none of which are widely cultivated for food use, as hosts for complex metabolic engineering of wax esters for lubricant applications. Lines producing wax esters >20% of total seed oil were generated for each crop and further improved for high temperature oxidative stability by down-regulation of fatty acid polyunsaturation. Field cultivation of optimized wax ester-producing crambe demonstrated commercial utility of these engineered crops and a path for sustainable production of other industrial oils in dedicated specialty oilseeds.",
author = "Zhu, {Li Hua} and Frans Krens and Smith, {Mark A.} and Xueyuan Li and Weicong Qi and {Van Loo}, {Eibertus N.} and Tim Iven and Ivo Feussner and Nazarenus, {Tara J.} and Dongxin Huai and Taylor, {David C.} and Zhou, {Xue Rong} and Green, {Allan G.} and Jay Shockey and Klasson, {K. Thomas} and Mullen, {Robert T.} and Bangquan Huang and Dyer, {John M.} and Cahoon, {Edgar B.}",
note = "Funding Information: This work is a part of EU-ICON project and financed by EU (FP7-KBBE-2007-1), The Swedish Research Council FORMAS, VINNOVA, Swedish Foundation for Strategic Research (SSF) and Swedish University of Agricultural Sciences. We wish to thank the ICON project coordinator Prof. Sten Stymne for his great support to this work and his valuable comments and discussion on the manuscript; The Rural Economy and Agricultural Societies for performing the crambe field trial; David Taylor and Mark Smith thank the Saskatchewan Agriculture Development Fund, Western Economic Partnership-Prairie Gold and the National Research Council Canada, for support of the carinata work. Xue-Rong Zhou and Allan Green thank New South Wales Department of Agriculture, Australia, for allowing collection of developing and mature seeds from jojoba plantations, and Edgar Cahoon thanks USDA-NIFA (Grant no. 2009-05988) and National Science Foundation (Plant Genome IOS-13-39385) for support of the camelina research.",
year = "2016",
month = feb,
day = "26",
doi = "10.1038/srep22181",
language = "English (US)",
volume = "6",
journal = "Scientific reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
}