Defective actin organization in cultured skin fibroblasts from individuals with inherited colon adenocarcinoma is not restored by addition of fibronectin

Levy Kopelovich; Clifford Murray; Hynda K. Kleinman; George R. Martin; Yuh Fukuda; Stephen I. Rennard

doi:10.1159/000163328

Defective actin organization in cultured skin fibroblasts from individuals with inherited colon adenocarcinoma is not restored by addition of fibronectin

Levy Kopelovich, Clifford Murray, Hynda K. Kleinman, George R. Martin, Yuh Fukuda, Stephen I. Rennard

Pulmonary, Critical Care, & Sleep Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Cultured skin fibroblasts from patients with hereditary adenomatosis of the colon and rectum (ACR) have a disorganized cytoskeleton. Since fibronectin has been found to restore normal cytoskeletal and cellular morphology to transformed cells, we investigated the fibronectin pattern in ACR cells. We found that although both fibronectin synthesis and binding to the cell surface were apparently normal, addition of fibronectin did not restore a normal distribution of actin cables to ACR cells. The data suggest that coupling of cell surface-bound fibronectin to the cytoskeleton might be constitutively defective in ACR cells.

Original language	English (US)
Pages (from-to)	314-321
Number of pages	8
Journal	Pathobiology
Volume	53
Issue number	6
DOIs	https://doi.org/10.1159/000163328
State	Published - 1985

Keywords

Cancer predisposition
Constitutive
Cultured human fibroblasts
Defective actin organization
Fibronectin

ASJC Scopus subject areas

Pathology and Forensic Medicine
Molecular Biology
Cell Biology

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10.1159/000163328

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title = "Defective actin organization in cultured skin fibroblasts from individuals with inherited colon adenocarcinoma is not restored by addition of fibronectin",

abstract = "Cultured skin fibroblasts from patients with hereditary adenomatosis of the colon and rectum (ACR) have a disorganized cytoskeleton. Since fibronectin has been found to restore normal cytoskeletal and cellular morphology to transformed cells, we investigated the fibronectin pattern in ACR cells. We found that although both fibronectin synthesis and binding to the cell surface were apparently normal, addition of fibronectin did not restore a normal distribution of actin cables to ACR cells. The data suggest that coupling of cell surface-bound fibronectin to the cytoskeleton might be constitutively defective in ACR cells.",

keywords = "Cancer predisposition, Constitutive, Cultured human fibroblasts, Defective actin organization, Fibronectin",

author = "Levy Kopelovich and Clifford Murray and Kleinman, {Hynda K.} and Martin, {George R.} and Yuh Fukuda and Rennard, {Stephen I.}",

year = "1985",

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T1 - Defective actin organization in cultured skin fibroblasts from individuals with inherited colon adenocarcinoma is not restored by addition of fibronectin

AU - Kopelovich, Levy

AU - Murray, Clifford

AU - Kleinman, Hynda K.

AU - Martin, George R.

AU - Fukuda, Yuh

AU - Rennard, Stephen I.

PY - 1985

Y1 - 1985

N2 - Cultured skin fibroblasts from patients with hereditary adenomatosis of the colon and rectum (ACR) have a disorganized cytoskeleton. Since fibronectin has been found to restore normal cytoskeletal and cellular morphology to transformed cells, we investigated the fibronectin pattern in ACR cells. We found that although both fibronectin synthesis and binding to the cell surface were apparently normal, addition of fibronectin did not restore a normal distribution of actin cables to ACR cells. The data suggest that coupling of cell surface-bound fibronectin to the cytoskeleton might be constitutively defective in ACR cells.

AB - Cultured skin fibroblasts from patients with hereditary adenomatosis of the colon and rectum (ACR) have a disorganized cytoskeleton. Since fibronectin has been found to restore normal cytoskeletal and cellular morphology to transformed cells, we investigated the fibronectin pattern in ACR cells. We found that although both fibronectin synthesis and binding to the cell surface were apparently normal, addition of fibronectin did not restore a normal distribution of actin cables to ACR cells. The data suggest that coupling of cell surface-bound fibronectin to the cytoskeleton might be constitutively defective in ACR cells.

KW - Cancer predisposition

KW - Constitutive

KW - Cultured human fibroblasts

KW - Defective actin organization

KW - Fibronectin

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Defective actin organization in cultured skin fibroblasts from individuals with inherited colon adenocarcinoma is not restored by addition of fibronectin

Abstract

Keywords

ASJC Scopus subject areas

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