Dendritic cells transfected with adenoviral vectors as vaccines

Joseph Senesac, Dmitry Gabrilovich, Samuel Pirruccello, James E. Talmadge

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Dendritic cells (DCs) are critical to the initiation of a T-cell response. They constitute the most potent antigen-presenting cell (APC) endowed with the unique capacity to stimulate an antigen-specific T-cell responses by naive T cells. Adenoviruses (Ad) have high transduction efficiency for many cell types including cells of hematopoietic origin independent of their mitotic status, and replication-defective Ad have demonstrated a safety profile clinically. Further, Ad vectors provide a high level of transgene expression, and Ad-transduced DCs can effectively present antigenic proteins. In this chapter, we outline a functionally closed, good manufacturing protocol for the differentiation of monocytes into DCs and transduction by Ad vectors. Basic functional and phenotypic release assays are provided, as well as contrasting research approaches for Ad-transduced DC-based vaccines.

Original languageEnglish (US)
Pages (from-to)97-118
Number of pages22
JournalMethods in Molecular Biology
Volume1139
DOIs
StatePublished - Jan 1 2014

Keywords

  • Adenovirus
  • Dendritic cells
  • Monocytes
  • Release assays
  • Vaccine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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