Dendritic polyglycerol with secondary amine shell as an efficient gene delivery vector with reduced toxicity

Yu Zhu, Stuart Hazeldine, Jing Li, David Oupický

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Dendritic polycation (PG-BEN) using polyglycerol as a core and secondary amine shell consisting of N1,N11-bisethylnorspermine (BEN) was synthesized. Polymers containing primary amines in the shell (PG-Nor and PG-NH2) were synthesized as controls to allow evaluation of the shell effect on physicochemical and transfection properties of the polymers. All studied polymers condensed DNA and formed polyplexes with sizes less than 110nm. PG-BEN and PG-Nor had a similar transfection activity that was fully comparable with that of the control polyethylenimine. Amongst the studied polymers, PG-BEN demonstrated the lowest cytotoxicity, suggesting that PG-BEN is a promising gene delivery vector with favorable transfection/toxicity profile.

Original languageEnglish (US)
Pages (from-to)940-947
Number of pages8
JournalPolymers for Advanced Technologies
Volume25
Issue number9
DOIs
StatePublished - Sep 2014

Keywords

  • Gene delivery
  • Polyamine
  • Polyglycerol
  • Polyplex
  • Transfection

ASJC Scopus subject areas

  • Polymers and Plastics

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