@inbook{476c6fcaa2a74475bd6dc92df9e058b0,
title = "Design and synthesis of fluorescent activity probes for protein phosphatases",
abstract = "Protein phosphatases act in concert with protein kinases to regulate and maintain the phosphoproteome. However, the catalog of chemical tools to directly monitor the enzymatic activity of phosphatases has lagged behind their kinase counterparts. In this chapter, we provide protocols for repurposing the phosphorylation-sensitive sulfonamido-oxine fluorophore known as Sox to afford direct activity probes for phosphatases. With validated activity probes in-hand, inhibitor screens can be conducted with recombinant enzyme and the role of phosphatases in cell signaling can be investigated in unfractionated cell lysates.",
keywords = "Biosensor, Cell signaling, Fluorescent probes, Phosphatase, Sensor",
author = "Casey, {Garrett R.} and Beck, {Jon R.} and Stains, {Cliff I.}",
note = "Funding Information: This work was supported by the NIH (R35GM119751). The content of this work is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.mie.2019.02.002",
language = "English (US)",
isbn = "9780128181195",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "29--53",
editor = "Shukla, {Arun K.}",
booktitle = "Chemical and Synthetic Biology Approaches To Understand Cellular Functions - Part B",
}