Design, synthesis and biological evaluation of sugar-derived esters, α-ketoesters and α-ketoamides as inhibitors for Mycobacterium tuberculosis antigen 85C

Aditya K. Sanki, Julie Boucau, Francis E. Umesiri, Donald R. Ronning, Steven J. Sucheck

Research output: Contribution to journalArticle

20 Scopus citations

Abstract

Peptide-based 1,2-dicarbonyl compounds have emerged as potent inhibitors for serine proteases. Herein, we have designed and synthesized d-arabinose and d-trehalose-based esters, α-ketoesters and α-ketoamides, and evaluated their inhibitory activity against Mycobacterium tuberculosis (Mtb) antigen 85C (ag85C), an acyltransferase in the serine hydrolase superfamily. In addition the compounds were evaluated for the ability to inhibit the growth of Mycobacterium smegmatis ATCC 14468, a non-pathogenic surrogate for Mtb. Among the synthetic analogs evaluated only the methyl ester 1 derived from d-arabinose was found to inhibit the acyltransferase activity of ag85C (IC50 = 25 mM). Based on this weak inhibitory activity it was not surprising that none of the compounds inhibits the growth of M. smegmatis. In spite of the weak inhibitory activity of 1, X-ray crystallography on crystals of ag85C soaked with 1 suggested the formation of a covalent ester adduct between 1 and the Ser124 side chain hydroxyl moiety found within the catalytic site of ag85C; however, some of the active site electron density appears to result from bound glycerol. The lack of activity associated with the α-ketoester and α-ketoamide derivatives of d-trehalose may be the result of intramolecular cyclization of the α-keto moiety with the nearby C-4/4′ hydroxyls leading to the formation of stable bicyclo-ester and amide derivatives.

Original languageEnglish (US)
Pages (from-to)945-956
Number of pages12
JournalMolecular BioSystems
Volume5
Issue number9
DOIs
StatePublished - 2009

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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