Detailed deletion mapping of chromosome 9p and p16 gene alterations in human borderline and invasive epithelial ovarian tumors

K. J. Rodabaugh, R. B. Biggs, J. A. Qureshi, A. J. Barrett, W. R. Welch, D. A. Bell, R. S. Berkowitz, S. C. Mok

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

We used PCR amplification of tandem repeats to study the pattern of allelic loss in borderline and invasive ovarian epithelial tumors using 12 primer pairs to generate a detailed deletion map of chromosome 9p. In the invasive ovarian carcinomas, there were three regions displaying high frequency of loss of heterozygosity (LOH) ranging from 31-38%. In contrast, LOH was a rare event among the borderline ovarian tumors, with one region revealing a rate of 20% and the remaining regions only 0-8% LOH. Therefore, allelic loss does not seem to be important for the development of borderline ovarian tumors. We also examined p16 gene expression and mutations in ovarian cancer cell lines and invasive and borderline ovarian tumor tissues. Southern blot analysis revealed no losses of the p16 gene in either the invasive or borderline ovarian tumors. However, the ovarian carcinoma cell lines showed a 50% homozygous deletion rate. SSCP analysis detected a mobility shift in only one (borderline) tumor. Since the primary invasive ovarian tumors did not show any deletions or mutations, it appears that p16 does not play a role in the pathogenesis of these tumors.

Original languageEnglish (US)
Pages (from-to)1249-1254
Number of pages6
JournalOncogene
Volume11
Issue number7
StatePublished - 1995

Keywords

  • Borderline ovarian tumor
  • Chromosome 9p
  • Loss of heterozygosity
  • Ovarian cancer
  • p16 gene

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Rodabaugh, K. J., Biggs, R. B., Qureshi, J. A., Barrett, A. J., Welch, W. R., Bell, D. A., Berkowitz, R. S., & Mok, S. C. (1995). Detailed deletion mapping of chromosome 9p and p16 gene alterations in human borderline and invasive epithelial ovarian tumors. Oncogene, 11(7), 1249-1254.