Detecting fish parvalbumin with commercial mouse monoclonal anti-frog parvalbumin IgG

Lingyun Chen, Sue L. Hefle, Steve L. Taylor, Ines Swoboda, Richard E. Goodman

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Parvalbumin is a calcium-binding muscle protein that is highly conserved across fish species and amphibians. It is the major cross-reactive allergen associated with both fish and frog allergy. We used two-dimensional electrophoretic and immunoblotting techniques to investigate the utility of a commercial monoclonal anti-frog parvalbumin IgG for detecting parvalbumin present in some commonly consumed fish species. The 2D electrophoresis and immunoblots revealed species-specific differences in proteins that appear to represent various numbers of isoforms of parvalbumin in carp (5), catfish (3), cod (1) and tilapia (2). No parvalbumin was detected in yellowfin tuna. Based on minor differences in relative intensities of protein staining and immunodetection, parvalbumin isoforms may have slight differences in the epitope region recognized by the anti-frog parvalbumin antibody. These results suggest that the frog anti-parvalbumin antibody can be used as a valuable tool to detect parvalbumins from the fish tested in this study, except yellowfin tuna.

Original languageEnglish (US)
Pages (from-to)5577-5582
Number of pages6
JournalJournal of Agricultural and Food Chemistry
Volume54
Issue number15
DOIs
StatePublished - Jul 26 2006

Keywords

  • 2D electrophoresis
  • Allergen
  • Antibody
  • Fish
  • Immunoblot
  • Parvalbumin

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

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