Detecting the presence of nora virus in Drosophila utilizing single fly RT-PCR

Bethany Munn; Brad Ericson; Darby J. Carlson; Kimberly A. Carlson

Detecting the presence of nora virus in Drosophila utilizing single fly RT-PCR

Bethany Munn, Brad Ericson, Darby J. Carlson, Kimberly A. Carlson

Research output: Contribution to journal › Article › peer-review

Abstract

A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in Drosophila. Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology students to gain relevant laboratory experience. The study can be easily adapted to best meet the needs of instructor and student, and provide exposure to PCR, work with Drosophila, data analysis, and molecular biology.

Original language	English (US)
Pages (from-to)	40-44
Number of pages	5
Journal	Bioscene
Volume	41
Issue number	2
State	Published - Dec 2015

Keywords

Drosophila
Nora virus
Single fly RT-PCR
Wild-caught flies

ASJC Scopus subject areas

Education
General Agricultural and Biological Sciences

Cite this

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abstract = "A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in Drosophila. Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology students to gain relevant laboratory experience. The study can be easily adapted to best meet the needs of instructor and student, and provide exposure to PCR, work with Drosophila, data analysis, and molecular biology.",

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language = "English (US)",

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AU - Munn, Bethany

AU - Ericson, Brad

AU - Carlson, Darby J.

AU - Carlson, Kimberly A.

PY - 2015/12

Y1 - 2015/12

N2 - A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in Drosophila. Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology students to gain relevant laboratory experience. The study can be easily adapted to best meet the needs of instructor and student, and provide exposure to PCR, work with Drosophila, data analysis, and molecular biology.

AB - A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in Drosophila. Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology students to gain relevant laboratory experience. The study can be easily adapted to best meet the needs of instructor and student, and provide exposure to PCR, work with Drosophila, data analysis, and molecular biology.

KW - Drosophila

KW - Nora virus

KW - Single fly RT-PCR

KW - Wild-caught flies

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JF - Bioscene

IS - 2

ER -

Detecting the presence of nora virus in Drosophila utilizing single fly RT-PCR

Abstract

Keywords

ASJC Scopus subject areas

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