Abstract
A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in Drosophila. Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology students to gain relevant laboratory experience. The study can be easily adapted to best meet the needs of instructor and student, and provide exposure to PCR, work with Drosophila, data analysis, and molecular biology.
Original language | English (US) |
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Pages (from-to) | 40-44 |
Number of pages | 5 |
Journal | Bioscene |
Volume | 41 |
Issue number | 2 |
State | Published - Dec 2015 |
Keywords
- Drosophila
- Nora virus
- Single fly RT-PCR
- Wild-caught flies
ASJC Scopus subject areas
- Education
- General Agricultural and Biological Sciences