Detecting the presence of nora virus in Drosophila utilizing single fly RT-PCR

Bethany Munn, Brad Ericson, Darby J. Carlson, Kimberly A. Carlson

Research output: Contribution to journalArticle

Abstract

A single fly RT-PCR protocol has recently been developed to detect the presence of the persistent, horizontally transmitted Nora virus in Drosophila. Wild-caught flies from Ohio were tested for the presence of the virus, with nearly one-fifth testing positive. The investigation presented can serve as an ideal project for biology students to gain relevant laboratory experience. The study can be easily adapted to best meet the needs of instructor and student, and provide exposure to PCR, work with Drosophila, data analysis, and molecular biology.

Original languageEnglish (US)
Pages (from-to)40-44
Number of pages5
JournalBioscene
Volume41
Issue number2
StatePublished - Dec 2015

Keywords

  • Drosophila
  • Nora virus
  • Single fly RT-PCR
  • Wild-caught flies

ASJC Scopus subject areas

  • Education
  • Agricultural and Biological Sciences(all)

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