Detection and identification of variola virus DNA in aerosol collection filters and/or liquids

Ted Hadfield; Victoria Olson; Douglas Anders; Linda Beck; Donna Boston; Kenneth Damer; Inger Damon; Matthew Davenport; Christina Egan; Steven Hinrichs; Penny Hitchcock; Anthony Hitchins; John Huggins; Mohamed Ibrahim; Paul Jackson; Liz Kerrigan; Karen Kesterson; Katalin Kiss; Angelo Madonna; Hermann Meyer; Denise Pettit; Frank Schaefer; Mark Scheckelhoff; Elizabeth Vitalis; Scott Coates

doi:10.5740/jaoac.int.SMPR2014.006

Detection and identification of variola virus DNA in aerosol collection filters and/or liquids

Ted Hadfield, Victoria Olson, Douglas Anders, Linda Beck, Donna Boston, Kenneth Damer, Inger Damon, Matthew Davenport, Christina Egan, Steven Hinrichs, Penny Hitchcock, Anthony Hitchins, John Huggins, Mohamed Ibrahim, Paul Jackson, Liz Kerrigan, Karen Kesterson, Katalin Kiss, Angelo Madonna, Hermann MeyerDenise Pettit, Frank Schaefer, Mark Scheckelhoff, Elizabeth Vitalis, Scott Coates

Research output: Contribution to journal › Article › peer-review

Abstract

The method of detection and identification of Variola virus DNA in aerosol collection filters and/or liquids is discussed. The analytical technique used is polymerase chain reaction (PCR) methods. The predetermined minimum level of an analyte, as specified by an expert committee, which must be detected by the candidate method at a specified probability of detection (POD) is necessary. The inclusivity panel shall include sequences from at least two representative strains from each major clade of Variola virus Any other strain with differences in the assay primer and/or probe target sequences based on bioinformatic analysis. Inclusion of all environmental panel organisms is not a requirement if a method developer provides appropriate justification that the intended use of the assay permits the exclusion of specific panel organisms. Justification for exclusion of any environmental panel organism(s) must be documented and submitted.

Original language	English (US)
Pages (from-to)	1046-1049
Number of pages	4
Journal	Journal of AOAC International
Volume	98
Issue number	4
DOIs	https://doi.org/10.5740/jaoac.int.SMPR2014.006
State	Published - Jul 1 2015

ASJC Scopus subject areas

General Medicine

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10.5740/jaoac.int.SMPR2014.006

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Hadfield, T., Olson, V., Anders, D., Beck, L., Boston, D., Damer, K., Damon, I., Davenport, M., Egan, C., Hinrichs, S., Hitchcock, P., Hitchins, A., Huggins, J., Ibrahim, M., Jackson, P., Kerrigan, L., Kesterson, K., Kiss, K., Madonna, A., ... Coates, S. (2015). Detection and identification of variola virus DNA in aerosol collection filters and/or liquids. Journal of AOAC International, 98(4), 1046-1049. https://doi.org/10.5740/jaoac.int.SMPR2014.006

Hadfield, T, Olson, V, Anders, D, Beck, L, Boston, D, Damer, K, Damon, I, Davenport, M, Egan, C, Hinrichs, S, Hitchcock, P, Hitchins, A, Huggins, J, Ibrahim, M, Jackson, P, Kerrigan, L, Kesterson, K, Kiss, K, Madonna, A, Meyer, H, Pettit, D, Schaefer, F, Scheckelhoff, M, Vitalis, E & Coates, S 2015, 'Detection and identification of variola virus DNA in aerosol collection filters and/or liquids', Journal of AOAC International, vol. 98, no. 4, pp. 1046-1049. https://doi.org/10.5740/jaoac.int.SMPR2014.006

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abstract = "The method of detection and identification of Variola virus DNA in aerosol collection filters and/or liquids is discussed. The analytical technique used is polymerase chain reaction (PCR) methods. The predetermined minimum level of an analyte, as specified by an expert committee, which must be detected by the candidate method at a specified probability of detection (POD) is necessary. The inclusivity panel shall include sequences from at least two representative strains from each major clade of Variola virus Any other strain with differences in the assay primer and/or probe target sequences based on bioinformatic analysis. Inclusion of all environmental panel organisms is not a requirement if a method developer provides appropriate justification that the intended use of the assay permits the exclusion of specific panel organisms. Justification for exclusion of any environmental panel organism(s) must be documented and submitted.",

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AU - Hadfield, Ted

AU - Olson, Victoria

AU - Anders, Douglas

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AU - Damer, Kenneth

AU - Damon, Inger

AU - Davenport, Matthew

AU - Egan, Christina

AU - Hinrichs, Steven

AU - Hitchcock, Penny

AU - Hitchins, Anthony

AU - Huggins, John

AU - Ibrahim, Mohamed

AU - Jackson, Paul

AU - Kerrigan, Liz

AU - Kesterson, Karen

AU - Kiss, Katalin

AU - Madonna, Angelo

AU - Meyer, Hermann

AU - Pettit, Denise

AU - Schaefer, Frank

AU - Scheckelhoff, Mark

AU - Vitalis, Elizabeth

AU - Coates, Scott

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Detection and identification of variola virus DNA in aerosol collection filters and/or liquids

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