Detection and quantification of plant sphingolipids by LC-MS

Jonathan E. Markham

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

Sphingolipids generate signals in plants in response to a variety of biotic and abiotic stresses. Measuring these signaling compounds is complicated by the heterogeneity of structures within the sphingolipid family and the comparatively low concentration of their metabolites in plant tissues. To date, the only method with the sensitivity, dynamic range, and specificity to measure all sphingolipids in a plant extract is liquid chromatography coupled to mass spectrometry. The drawback of this method is the cost of the hardware, the expertise in mass spectrometry required to critically assess the outcome and the lack of suitable standards for accurate quantitative analysis. The goal of this chapter is to assist researchers in setting up experiments to measure sphingolipids and explain some of the pitfalls and solutions along the way.

Original languageEnglish (US)
Title of host publicationPlant Lipid Signaling Protocols
PublisherHumana Press Inc.
Pages93-101
Number of pages9
ISBN (Print)9781627034005
DOIs
StatePublished - 2013

Publication series

NameMethods in Molecular Biology
Volume1009
ISSN (Print)1064-3745

Keywords

  • Ceramide
  • HPLC
  • Long-chain base
  • Long-chain base phosphate
  • Mass spectrometry
  • Sphingolipid
  • Sphingosine

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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