TY - JOUR
T1 - Detection of cardiac signaling in the injured and hypertrophied heart.
AU - Si, Xiaoning
AU - Rahmani, Maziar
AU - Yuan, J.
AU - Luo, Honglin
N1 - Copyright:
This record is sourced from MEDLINE/PubMed, a database of the U.S. National Library of Medicine
PY - 2005
Y1 - 2005
N2 - Cardiac hypertrophy is a compensatory response to a variety of physiological or pathological stimuli. However, prolonged hypertrophic responses may eventually lead to heart failure, arrhythmia, and sudden death. A number of intracellular signaling pathways have been implicated to play a critical role in the regulation of cardiac hypertrophy. In this chapter, the mitogen-activated protein kinase signaling pathway is used to illustrate conventional assays to detect the expression, phosphorylation, and activation of signaling proteins during cardiac hypertrophy, including Western blot, immunohistochemical staining, and immune complex kinase assays. Newly emerging techniques for analyzing cell signaling are also discussed in this chapter. Identifying and characterizing the expression and activation of these signaling proteins will provide important insights into the mechanisms that regulate hypertrophic cell growth and assist in development of new therapeutic approaches to limit cardiac hypertrophy.
AB - Cardiac hypertrophy is a compensatory response to a variety of physiological or pathological stimuli. However, prolonged hypertrophic responses may eventually lead to heart failure, arrhythmia, and sudden death. A number of intracellular signaling pathways have been implicated to play a critical role in the regulation of cardiac hypertrophy. In this chapter, the mitogen-activated protein kinase signaling pathway is used to illustrate conventional assays to detect the expression, phosphorylation, and activation of signaling proteins during cardiac hypertrophy, including Western blot, immunohistochemical staining, and immune complex kinase assays. Newly emerging techniques for analyzing cell signaling are also discussed in this chapter. Identifying and characterizing the expression and activation of these signaling proteins will provide important insights into the mechanisms that regulate hypertrophic cell growth and assist in development of new therapeutic approaches to limit cardiac hypertrophy.
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U2 - 10.1007/978-1-59259-879-3_19
DO - 10.1007/978-1-59259-879-3_19
M3 - Article
C2 - 16010025
AN - SCOPUS:23044499489
SN - 1543-1894
VL - 112
SP - 291
EP - 303
JO - Methods in molecular medicine
JF - Methods in molecular medicine
ER -