Detection of circulating antibodies to malondialdehyde-acetaldehyde adducts in ethanol-fed rats

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70 Scopus citations

Abstract

Background and Aims: Malondialdehyde and acetaldehyde react together with proteins and form hybrid protein conjugates designated as MAA adducts, which have been detected in livers of ethanol-fed rats. The aim of this study was to examine the immune response to MAA adducts and other aidehyde adducts during long-term ethanol exposure. Methods: Rats were pairfed for 7 months with a liquid diet containing either ethanol or isocaloric carbohydrate. Circulating antibody titers against MAA adducts and acetaldehyde adducts were measured and characterized in these animals. Results: A significant increase in antibody titers against MAA-adducted proteins was observed in the ethanol- fed animals. Competitive inhibitions of antibody binding indicated that the circulating antibodies against MAA-modified proteins in the ethanol-fed rats recognized mainly a specific, chemically defined MAA epitope. Antibody titers to reduced and nonreduced acetaldehyde adducts were very low, and no significant differences were observed between ethanol-fed and control animals. Significant plasma immunoreactivity to not only MAA-adducted but also unmodified rat liver proteins (cytosol, microsomes, and especially plasma membrane) were also observed in the ethanol-fed rats. Conclusions: Long-term ethanol feeding generates circulating antibodies not only against MAA epitopes but possibly also against unmodified, native (self) protein epitopes, suggesting that MAA adducts could trigger harmful autoimmune responses.

Original languageEnglish (US)
Pages (from-to)686-692
Number of pages7
JournalGastroenterology
Volume115
Issue number3
DOIs
StatePublished - 1998

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

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