Detection of endoproteinases in polyacrylamide gels

Gary R. Petersen, James L. Van Etten

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

A simple and versatile method was developed for detecting endoproteinases by nondenaturing polyacrylamide gel electrophoresis. A vertical strip of the resolving gel is subjected horizontally to a second electrophoresis in the presence of a substrate protein and sodium dodecyl sulfate (SDS). The location of proteolytic activity in the original gel strip is revealed by the disappearance of substrate and the appearance of proteolytic products (due to limited proteolysis) in the second gel. The pattern of peptide fragments produced permits the identification of the protease responsible.

Original languageEnglish (US)
Pages (from-to)433-435
Number of pages3
JournalELECTROPHORESIS
Volume4
Issue number6
DOIs
StatePublished - Feb 1983

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Detection of endoproteinases in polyacrylamide gels'. Together they form a unique fingerprint.

Cite this