Detection of phagocyte-derived free radicals with spin trapping techniques: Effect of temperature and cellular metabolism

Gerald M. Rosen, Bradley E. Britigan, Myron S. Cohen, Sharon P. Ellington, Michael J. Barber

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Human neutrophils activated with either particulate or soluble stimuli generate oxygen-centered free radicals which are detected by spin trapping in conjunction with electron spin resonance (ESR) spectroscopy. We investigated the effect of temperature on ESR spectra resulting from stimulation of human neutrophils with phorbol myristate acetate (PMA) or opsonized zymosan in the presence of the spin trap, 5,5-dimethyl-1-pyrroline 1-oxide (DMPO). At 20°C with either stimuli, neutrophil superoxide production was manifested predominantely as the superoxide spin-trapped adduct, 5,5-dimethyl-5-hydroperoxy-1-pyrrolidinyloxy (DMPO-OOH). In contrast, at 37°C, the hydroxyl spin-trapped adduct, 2,2-dimethyl-5-hydroxy-1-pyrrolidinyloxy (DMPO-OH) was dominant. No evidence of hydroxyl radical (defined as the methyl spin-trapped adduct, 2,2,5-trimethyl-1-pyrrolidinyloxy, DMPO-CH3) was observed, suggesting that elevated temperatures increased the rate of DMPO-OOH conversion to DMPO-OH. In addition, the elevated temperature activated a neutrophil reductase which accelerated the rate of DMPO-OH reduction to its corresponding hydroxylamine, 2,2-dimethyl-5-hydroxy-1-hydroxypyrrolidine. This bioreduction was dependent upon the presence of both superoxide and a phagocyte-derived factor (possibly a thiol) released into the surrounding media.

Original languageEnglish (US)
Pages (from-to)236-241
Number of pages6
JournalBBA - Molecular Cell Research
Volume969
Issue number3
DOIs
StatePublished - May 13 1988

Keywords

  • (Human)
  • ESR
  • Free radical
  • Phagocyte stimulation
  • Superoxide generation
  • Temperature dependence

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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