Detection of trichloroethylene-protein adducts in rat liver and plasma

N. Christine Halmes, Everett J. Perkins, David C. McMillan, Neil R. Pumford

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Trichloroethylene is an industrial chemical with widespread occupational exposure and is a major environmental contaminant. In a Western blot using antiserum that recognizes trichloroethylene covalently bound to protein, a single 50 kDa microsomal adduct was detected in the livers of trichloroethylene-treated Sprague-Dawley rats. To determine if trichloroethylene-protein adducts could be detected in blood, plasma proteins were immunoaffinity purified using an antidichloroacetyl column. A single 50 kDa protein was detected in the affinity-purified fraction in a Western blot using dichloroacetyl antiserum. This protein was also immunochemically reactive with anticytochrome P450 2E1 antibodies. The 50 kDa trichloroethylene-protein adduct may be formed in the liver and released into the blood following exposure to trichloroethylene. The significance of adduct formation with respect to trichloroethylene toxicity remains to be established; however, the data suggest that this approach may be useful in the investigation of trichloroethylene-protein adducts and adverse effects following exposure.

Original languageEnglish (US)
Pages (from-to)187-194
Number of pages8
JournalToxicology Letters
Volume92
Issue number3
DOIs
StatePublished - Aug 22 1997

Keywords

  • Autoimmunity
  • Cancer
  • Cytochrome P450 2E1
  • Human
  • Protein-adduct
  • Rat
  • Toxicity
  • Trichloroethylene (1,1,2-trichloroethene)

ASJC Scopus subject areas

  • Toxicology

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