Determination of DNA cooperativity factor

B. R. Amirikyan, A. V. Vologodskii, Yu L. Lyubchenko

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The paper presents measurements of the difference in the melting temperature of a Co1E1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for 0.2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5×10-5 for the cooperatively factor σ. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

Original languageEnglish (US)
Pages (from-to)5469-5482
Number of pages14
JournalNucleic acids research
Volume9
Issue number20
DOIs
StatePublished - Oct 24 1981

ASJC Scopus subject areas

  • Genetics

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