Determination of DNA cooperativity factor

B. R. Amirikyan; A. V. Vologodskii; Yu L. Lyubchenko

doi:10.1093/nar/9.20.5469

Determination of DNA cooperativity factor

B. R. Amirikyan, A. V. Vologodskii, Yu L. Lyubchenko

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38 Scopus citations

Abstract

The paper presents measurements of the difference in the melting temperature of a Co1E1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for 0.2 M Na⁺ (the conditions for fully reversible melting) yielded the value of 2.5-5×10^-5 for the cooperatively factor σ. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

Original language	English (US)
Pages (from-to)	5469-5482
Number of pages	14
Journal	Nucleic acids research
Volume	9
Issue number	20
DOIs	https://doi.org/10.1093/nar/9.20.5469
State	Published - Oct 24 1981
Externally published	Yes

ASJC Scopus subject areas

Genetics

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AU - Vologodskii, A. V.

AU - Lyubchenko, Yu L.

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N2 - The paper presents measurements of the difference in the melting temperature of a Co1E1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for 0.2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5×10-5 for the cooperatively factor σ. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

AB - The paper presents measurements of the difference in the melting temperature of a Co1E1 DNA region when it is located inside the DNA helix and at its end. A direct comparison of calculations based on the rigorous theory of helix-coil transition with experimental data for 0.2 M Na+ (the conditions for fully reversible melting) yielded the value of 2.5-5×10-5 for the cooperatively factor σ. We discuss the reversibility of DNA melting and the possibility of applying the "all-or-nothing" concept to the melting of DNA regions.

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JO - Nucleic acids research

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Determination of DNA cooperativity factor

Abstract

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