TY - JOUR
T1 - Determination of isomeric dibenzo[a,l]pyreneadenine adducts by six different tandem mass spectrometric experiments
AU - Byun, Jaeman
AU - Gooden, Jonathon
AU - Ramanathan, Ragulan
AU - Li, Kai Ming
AU - Cavalieri, Ercole L.
AU - Gross, Michael L.
N1 - Funding Information:
This research was supported by the National Institutes of Health grant no. 1P01CA49210 and grant no. 2P41RR00954. The authors thank scientists at Finnigan, Micromass, and Sciex for assisting with data acquisition by triple-quadrupole mass spectrometry.
PY - 1997/9
Y1 - 1997/9
N2 - Six different tandem-mass-spectrometric experiments were evaluated to assess their ability to distinguish four isomeric, carcinogen-modified adenines. Metastable-ion dissociations and high-energy collisional activated decompositions (HE CAD) of fast-atom bombardment (FAB)-produced ions were followed on a four-sector tandem mass spectrometer. Precursor ions were also produced by electrospray on the four-sector instrument, and they were submitted to both high-energy and electrospray ionization (ESI)-source CAD. We contrasted these means of activation with low-energy (LE) CAD of electrospray-produced ions on a triple quadrupole instrument and with post- source decompositions (PSD) of ions produced by matrix-assisted laser desorption ionization (MALDI). The latter experiment was conducted with a time-of-flight mass spectrometer. The four subject molecules were isomeric dibenzo-[a,l]pyrene (DB[a,l]P) adenine adducts, in which N1, N3, N7, and N6 positions were substituted. We used similarity indicies (SI), which were calculated from the relative abundances of common fragment ions for the isomers, to confirm quantitatively that HE GAD of FAB and ESI-produced ions can distinguish two of three, and that LE CAD and MALDI-PSD can distinguish three of the three isomers that give qualitatively identical product-ion spectra. The results also show that LE CAD and MALDI-PSD have comparable discriminating capability for these three DBP adducts.
AB - Six different tandem-mass-spectrometric experiments were evaluated to assess their ability to distinguish four isomeric, carcinogen-modified adenines. Metastable-ion dissociations and high-energy collisional activated decompositions (HE CAD) of fast-atom bombardment (FAB)-produced ions were followed on a four-sector tandem mass spectrometer. Precursor ions were also produced by electrospray on the four-sector instrument, and they were submitted to both high-energy and electrospray ionization (ESI)-source CAD. We contrasted these means of activation with low-energy (LE) CAD of electrospray-produced ions on a triple quadrupole instrument and with post- source decompositions (PSD) of ions produced by matrix-assisted laser desorption ionization (MALDI). The latter experiment was conducted with a time-of-flight mass spectrometer. The four subject molecules were isomeric dibenzo-[a,l]pyrene (DB[a,l]P) adenine adducts, in which N1, N3, N7, and N6 positions were substituted. We used similarity indicies (SI), which were calculated from the relative abundances of common fragment ions for the isomers, to confirm quantitatively that HE GAD of FAB and ESI-produced ions can distinguish two of three, and that LE CAD and MALDI-PSD can distinguish three of the three isomers that give qualitatively identical product-ion spectra. The results also show that LE CAD and MALDI-PSD have comparable discriminating capability for these three DBP adducts.
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U2 - 10.1016/S1044-0305(97)00125-6
DO - 10.1016/S1044-0305(97)00125-6
M3 - Article
AN - SCOPUS:0031238827
SN - 1044-0305
VL - 8
SP - 977
EP - 986
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 9
ER -