Determination of lamivudine in plasma, amniotic fluid, and rat tissues by liquid chromatography

Yazen Alnouti, Catherine A. White, Michael G. Bartlett

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

An HPLC method for the quantification of lamivudine (3TC) in rat plasma, amniotic fluid, placental and fetal tissues has been developed, validated and applied to the study of the placental transport of this drug in the pregnant rat. Placental and fetal tissues were processed using liquid-liquid extraction enhanced by salting out the sample using a saturated solution of ammonium sulfate. Plasma and amniotic fluid samples were processed by protein precipitation using 2M perchloric acid. Reverse phase chromatography was performed using a phenyl column (5μm, 150mm×2mm i.d.) under a flow rate of 0.2ml/min. The mobile phase consisted of 5% methanol in 20mM dibasic phosphate buffer (pH 6). The method was validated over the range from 0.1 to 50μg/ml for plasma and amniotic fluid and 0.2-50μg/ml for the placental and fetal tissues.

Original languageEnglish (US)
Pages (from-to)279-284
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume803
Issue number2
DOIs
StatePublished - Apr 25 2004

Keywords

  • Lamivudine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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