Abstract
An HPLC method for the quantification of lamivudine (3TC) in rat plasma, amniotic fluid, placental and fetal tissues has been developed, validated and applied to the study of the placental transport of this drug in the pregnant rat. Placental and fetal tissues were processed using liquid-liquid extraction enhanced by salting out the sample using a saturated solution of ammonium sulfate. Plasma and amniotic fluid samples were processed by protein precipitation using 2M perchloric acid. Reverse phase chromatography was performed using a phenyl column (5μm, 150mm×2mm i.d.) under a flow rate of 0.2ml/min. The mobile phase consisted of 5% methanol in 20mM dibasic phosphate buffer (pH 6). The method was validated over the range from 0.1 to 50μg/ml for plasma and amniotic fluid and 0.2-50μg/ml for the placental and fetal tissues.
Original language | English (US) |
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Pages (from-to) | 279-284 |
Number of pages | 6 |
Journal | Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences |
Volume | 803 |
Issue number | 2 |
DOIs | |
State | Published - Apr 25 2004 |
Externally published | Yes |
Keywords
- Lamivudine
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Clinical Biochemistry
- Cell Biology