Determination of riboflavin and flavocoenzymes in human blood plasma by high-performance liquid chromatography

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

A high-performance liquid chromatographic method for determining riboflavin, flavin adenine dinucleotide, and flavin mononucleotide in human blood plasma is presented. Flavocoenzymes are determined as flavin mononucleotide after acid hydrolysis of flavin adenine dinucleotide. Metabolites are separated by reversed-phase column chromatography and quantified by their native fluorescence. Criteria of quality are (riboflavin/flavocoenzymes): coefficients of variation 2.8/4.6% (intra-assay) and 2.8/4.4% (inter-assay); recovery 82.4/94.4%; detection limit <3.0/9.0 nmol/l. Because sample preparation requires only few steps, and the retention times are short (<5 min), this method is recommended for use in routine analysis.

Original languageEnglish (US)
Pages (from-to)224-226
Number of pages3
JournalAnnals of Nutrition and Metabolism
Volume39
Issue number4
DOIs
StatePublished - 1995
Externally publishedYes

Keywords

  • Flavin adenine dinucleotide
  • Flavin mononucleotide
  • High-performance liquid chromatography
  • Human blood plasma
  • Riboflavin

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

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