Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization

Mark S. Schmidt; Thomas E. Prisinzano; Kevin Tidgewell; Wayne Harding; Eduardo R. Butelman; Mary J. Kreek; Daryl J. Murry

doi:10.1016/j.jchromb.2004.12.041

Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization

Mark S. Schmidt, Thomas E. Prisinzano, Kevin Tidgewell, Wayne Harding, Eduardo R. Butelman, Mary J. Kreek, Daryl J. Murry

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d₃-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.

Original language	English (US)
Pages (from-to)	221-225
Number of pages	5
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	818
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2004.12.041
State	Published - Apr 25 2005
Externally published	Yes

Keywords

APCT
Analysis
Body fluids
LC-MS
Metabolism
Plasma
Salvinorin
Salvinorin A
Urine

ASJC Scopus subject areas

Analytical Chemistry
Biochemistry
Clinical Biochemistry
Cell Biology

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10.1016/j.jchromb.2004.12.041

Cite this

Schmidt, M. S., Prisinzano, T. E., Tidgewell, K., Harding, W., Butelman, E. R., Kreek, M. J., & Murry, D. J. (2005). Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 818(2), 221-225. https://doi.org/10.1016/j.jchromb.2004.12.041

Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization. / Schmidt, Mark S.; Prisinzano, Thomas E.; Tidgewell, Kevin et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 818, No. 2, 25.04.2005, p. 221-225.

Research output: Contribution to journal › Article › peer-review

Schmidt, MS, Prisinzano, TE, Tidgewell, K, Harding, W, Butelman, ER, Kreek, MJ & Murry, DJ 2005, 'Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 818, no. 2, pp. 221-225. https://doi.org/10.1016/j.jchromb.2004.12.041

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title = "Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization",

abstract = "Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.",

keywords = "APCT, Analysis, Body fluids, LC-MS, Metabolism, Plasma, Salvinorin, Salvinorin A, Urine",

author = "Schmidt, {Mark S.} and Prisinzano, {Thomas E.} and Kevin Tidgewell and Wayne Harding and Butelman, {Eduardo R.} and Kreek, {Mary J.} and Murry, {Daryl J.}",

note = "Funding Information: Studies were supported in part by NIH-NIDA grants DA11113 (ERB) and DA05130 and DA00049 (MJK).",

year = "2005",

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doi = "10.1016/j.jchromb.2004.12.041",

language = "English (US)",

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AU - Schmidt, Mark S.

AU - Prisinzano, Thomas E.

AU - Tidgewell, Kevin

AU - Harding, Wayne

AU - Butelman, Eduardo R.

AU - Kreek, Mary J.

AU - Murry, Daryl J.

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Y1 - 2005/4/25

N2 - Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.

AB - Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.

KW - APCT

KW - Analysis

KW - Body fluids

KW - LC-MS

KW - Metabolism

KW - Plasma

KW - Salvinorin

KW - Salvinorin A

KW - Urine

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JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

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Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization

Abstract

Keywords

ASJC Scopus subject areas

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