Determination of Salvinorin A in body fluids by high performance liquid chromatography-atmospheric pressure chemical ionization

Mark S. Schmidt, Thomas E. Prisinzano, Kevin Tidgewell, Wayne Harding, Eduardo R. Butelman, Mary J. Kreek, Daryl J. Murry

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Salvinorin A was quantitated in human and rhesus monkey plasma, rhesus monkey cerebrospinal fluid, and human urine by negative ion LC-MS/APCI. The method for Salvinorin A has been fully validated, the LLOQ using FDA guidelines is 2 ng/mL for 0.5 mL plasma samples. The linear range was from 2 to 1000 ng/mL. Several derivatives in the Salvinorin family can also be analyzed by this method; d3-Salvinorin A was prepared and used as internal standard. The metabolite Salvinorin B can be semi quantitatively determined. The method has been used to establish that Salvinorin B is the principal metabolite of Salvinorin A ex vivo and to establish the analytical method to study in vivo samples.

Original languageEnglish (US)
Pages (from-to)221-225
Number of pages5
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume818
Issue number2
DOIs
StatePublished - Apr 25 2005

Keywords

  • APCT
  • Analysis
  • Body fluids
  • LC-MS
  • Metabolism
  • Plasma
  • Salvinorin
  • Salvinorin A
  • Urine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Cell Biology

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