A radiochemical separation was developed for the determination of mercury in biological fluids and tissues. Mercury-197 was effectively separated from 82Br and other spectral interferences employing an oxidative digestion using concentrated sulfuric acid and potassium permanganate with separation of the mercury tetrahedral chloride complex on Dowex anion-exchange resin. The developed procedure gave quantitative recoveries for mercury in whole blood (99% for methylmercury and 101% for inorganic mercury). Mercury concentrations of NIST SRM 1577b and NIST SRM 2672a; both Elevated and Low level; were also determined and found to be in close agreement with reported values. A procedure to correct for the interference of 198Au was also reported.
- Biological matrices
- Mercury analysis
- Radiochemical separation
ASJC Scopus subject areas
- Physical and Theoretical Chemistry