Abstract
An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.
Original language | English (US) |
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Pages (from-to) | 540-543 |
Number of pages | 4 |
Journal | Journal of Pharmaceutical and Biomedical Analysis |
Volume | 41 |
Issue number | 2 |
DOIs | |
State | Published - May 3 2006 |
Externally published | Yes |
Keywords
- APCI
- Analysis
- Infant
- LC-MS
- Pediatric
- Plasma
- Vinblastine
- Vincristine
- Vinorelbine
ASJC Scopus subject areas
- Analytical Chemistry
- Pharmaceutical Science
- Drug Discovery
- Spectroscopy
- Clinical Biochemistry