Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

Mark S. Schmidt, Rong Huang, Robert J. Classon, Daryl J. Murry

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.

Original languageEnglish (US)
Pages (from-to)540-543
Number of pages4
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume41
Issue number2
DOIs
StatePublished - May 3 2006

Keywords

  • APCI
  • Analysis
  • Infant
  • LC-MS
  • Pediatric
  • Plasma
  • Vinblastine
  • Vincristine
  • Vinorelbine

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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