Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

Mark S. Schmidt; Rong Huang; Robert J. Classon; Daryl J. Murry

doi:10.1016/j.jpba.2005.11.039

Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

Mark S. Schmidt, Rong Huang, Robert J. Classon, Daryl J. Murry

Research output: Contribution to journal › Article › peer-review

16 Scopus citations

Abstract

An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.

Original language	English (US)
Pages (from-to)	540-543
Number of pages	4
Journal	Journal of Pharmaceutical and Biomedical Analysis
Volume	41
Issue number	2
DOIs	https://doi.org/10.1016/j.jpba.2005.11.039
State	Published - May 3 2006
Externally published	Yes

Keywords

APCI
Analysis
Infant
LC-MS
Pediatric
Plasma
Vinblastine
Vincristine
Vinorelbine

ASJC Scopus subject areas

Analytical Chemistry
Pharmaceutical Science
Drug Discovery
Spectroscopy
Clinical Biochemistry

Access to Document

10.1016/j.jpba.2005.11.039

Cite this

@article{ecf5df22e37c46999d59a69bca1269bb,

title = "Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy",

abstract = "An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.",

keywords = "APCI, Analysis, Infant, LC-MS, Pediatric, Plasma, Vinblastine, Vincristine, Vinorelbine",

author = "Schmidt, {Mark S.} and Rong Huang and Classon, {Robert J.} and Murry, {Daryl J.}",

note = "Funding Information: This work was supported by The Holden Comprehensive Cancer Center Cancer Center Support Grant 5 P30 CA86862-03 at the University of Iowa.",

year = "2006",

month = may,

day = "3",

doi = "10.1016/j.jpba.2005.11.039",

language = "English (US)",

volume = "41",

pages = "540--543",

journal = "Journal of Pharmaceutical and Biomedical Analysis",

issn = "0731-7085",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

AU - Schmidt, Mark S.

AU - Huang, Rong

AU - Classon, Robert J.

AU - Murry, Daryl J.

N1 - Funding Information: This work was supported by The Holden Comprehensive Cancer Center Cancer Center Support Grant 5 P30 CA86862-03 at the University of Iowa.

PY - 2006/5/3

Y1 - 2006/5/3

N2 - An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.

AB - An LC-MS method using APCI has been developed and validated for the determination of the anticancer drug vincristine in human plasma, using vinblastine as internal standard. Following solid-phase extraction (SPE) of the sample, the lower limit of quantitation (LLOQ) was 0.18 ng/ml, the lower limit of detection was 0.09 ng/ml, and the linear calibration range was 0.18-180 ng/ml. This method has been used to measure plasma concentrations of vincristine from 0.08 to 24 h post bolus in 29 infants as part of a pharmacokinetic study. Concentrations of vincristine at 24 h were 0.2-1.36 ng/ml.

KW - APCI

KW - Analysis

KW - Infant

KW - LC-MS

KW - Pediatric

KW - Plasma

KW - Vinblastine

KW - Vincristine

KW - Vinorelbine

UR - http://www.scopus.com/inward/record.url?scp=33645959550&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645959550&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2005.11.039

DO - 10.1016/j.jpba.2005.11.039

M3 - Article

C2 - 16414232

AN - SCOPUS:33645959550

VL - 41

SP - 540

EP - 543

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

IS - 2

ER -

Determination of vincristine in infant plasma by liquid chromatography-atmospheric pressure chemical ionization-mass spectroscopy

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this