@article{9dd11ae2ce0141848869808d2dacf0d3,
title = "Developing a new treatment for superficial fungal infection using antifungal Collagen-HSAF dressing",
abstract = "Fungal pathogens are common causes of superficial clinical infection. Their increasing drug resistance gradually makes existing antifungal drugs ineffective. Heat stable antifungal factor (HSAF) is a novel antifungal natural product with a unique structure. However, the application of HSAF has been hampered by very low yield in the current microbial producers and from extremely poor solubility in water and common solvents. In this study, we developed an effective mode of treatment applying HSAF to superficial fungal infections. The marine-derived Lysobacter enzymogenes YC36 contains the HSAF biosynthetic gene cluster, which we activated by the interspecific signaling molecule indole. An efficient extraction strategy was used to significantly improve the purity to 95.3%. Scanning electron microscopy images revealed that the Type I collagen-based HSAF (Col-HSAF) has a transparent appearance and good physical properties, and the in vitro sustained-release effect of HSAF was maintained for more than 2 weeks. The effective therapeutic concentration of Col-HSAF against superficial fungal infection was explored, and Col-HSAF showed good biocompatibility, lower clinical scores, mild histological changes, and antifungal capabilities in animals with Aspergillus fumigatus keratitis and cutaneous candidiasis. In conclusion, Col-HSAF is an antifungal reagent with significant clinical value in the treatment of superficial fungal infections.",
keywords = "A. fumigatus keratitis, antifungal dressing, cutaneous candidiasis, heat stable antifungal factor (HSAF), superficial fungal infections",
author = "Jing Zhong and Xiayi Yan and Xin Zuo and Xuan Zhao and Jiahui Yang and Qin Dou and Lulu Peng and Yuxiang Zhu and Yichen Xiao and Zeran Bian and Dalian He and Qiushuang Xu and Stephen Wright and Yaoyao Li and Liangcheng Du and Yan Wang and Jin Yuan",
note = "Funding Information: This work was supported by the National Natural Science Foundation of China (no. 81870633, no. 81670826), the open research funds of the State Key Laboratory of Ophthalmology (no. 303060202400368), Guangdong Guangdong‐Guangzhou Joint Fund Youth Project (no. 2019A1515110821), the Marine S&T Fund of Shandong Province for the Pilot National Laboratory for Marine Science and Technology (Qingdao) (no. 2018SDKJ0406‐4), the National Natural Science Foundation of China (no. 31870023), and the Young Taishan Scholars Program of Shandong Province (no. tsqn202103029). Funding Information: Guangdong Guangdong‐Guangzhou Joint Fund Youth Project, Grant/Award Number: 2019A1515110821; National Natural Science Foundation of China, Grant/Award Numbers: 31870023, 81670826, 81870633, 82101086; the Marine S&T Fund of Shandong Province for the Pilot National Laboratory for Marine Science and Technology (Qingdao), Grant/Award Number: 2018SDKJ0406‐4; the open research funds of the State Key Laboratory of Ophthalmology, Grant/Award Number: 303060202400368; the Young Taishan Scholars Program of Shandong Province, Grant/Award Number: tsqn202103029 Funding information A. fumigatus Funding Information: This work was supported by the National Natural Science Foundation of China (no. 81870633, no. 81670826), the open research funds of the State Key Laboratory of Ophthalmology (no. 303060202400368), Guangdong Guangdong-Guangzhou Joint Fund Youth Project (no. 2019A1515110821), the Marine S&T Fund of Shandong Province for the Pilot National Laboratory for Marine Science and Technology (Qingdao) (no. 2018SDKJ0406-4), the National Natural Science Foundation of China (no. 31870023), and the Young Taishan Scholars Program of Shandong Province (no. tsqn202103029). Publisher Copyright: {\textcopyright} 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.",
year = "2022",
month = sep,
doi = "10.1002/btm2.10304",
language = "English (US)",
volume = "7",
journal = "Bioengineering and Translational Medicine",
issn = "2380-6761",
publisher = "John Wiley & Sons Inc.",
number = "3",
}