Developing defined and scalable 3D culture systems for culturing human pluripotent stem cells at high densities

Yuguo Lei, Daeun Jeong, Jifang Xiao, David V. Schaffer

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

Human pluripotent stem cells (hPSCs) - including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) - are very promising candidates for cell therapies, tissue engineering, high throughput pharmacology screens, and toxicity testing. These applications require large numbers of high quality cells; however, scalable production of human pluripotent stem cells and their derivatives at a high density and under well-defined conditions has been a challenge. We recently reported a simple, efficient, fully defined, scalable, and good manufacturing practice (GMP) compatible 3D culture system based on a thermoreversible hydrogel for hPSC expansion and differentiation. Here, we describe additional design rationale and characterization of this system. For instance, we have determined that culturing hPSCs as a suspension in a liquid medium can exhibit lower volumetric yields due to cell agglomeration and possible shear force-induced cell loss. By contrast, using hydrogels as 3D scaffolds for culturing hPSCs reduces aggregation and may insulate from shear forces. Additionally, hydrogel-based 3D culture systems can support efficient hPSC expansion and differentiation at a high density if compatible with hPSC biology. Finally, there are considerable opportunities for future development to further enhance hydrogel-based 3D culture systems for producing hPSCs and their progeny.

Original languageEnglish (US)
Pages (from-to)172-183
Number of pages12
JournalCellular and Molecular Bioengineering
Volume7
Issue number2
DOIs
StatePublished - Jun 2014

Keywords

  • 3D culture system
  • Human embryonic stem cells
  • Induced pluripotent stem cells
  • Thermoreversible hydrogel

ASJC Scopus subject areas

  • Modeling and Simulation
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Developing defined and scalable 3D culture systems for culturing human pluripotent stem cells at high densities'. Together they form a unique fingerprint.

Cite this