Development of a direct sampling method for verifying the cleanliness of equipment shared with peanut products

Vicki Schlegel, Angeline Yong, Swee Yee Foo

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

A direct sampling method was developed to verify the cleanliness of stainless steel equipment used to produce peanut products. The protocol consisted of sampling the product-contact area with two Critical Swab® flat zapped-head foam swabs pre-moistened with 20 mM phosphate, pH 7.2 (PBS), mixing each swab in PBS for 1 h, and analyzing the extract for peanut residues. Quantitation of peanut residues was accomplished with a non-specific assay while SDS-PAGE was used to ensure the recovery of the allergen, Ara h 1. This sampling approach was able to recover 90-95% of the peanut proteins at sub-microgram levels and Ara h 1 at low nanogram levels directly from a stainless steel surface.

Original languageEnglish (US)
Pages (from-to)1494-1500
Number of pages7
JournalFood Control
Volume18
Issue number12
DOIs
StatePublished - Dec 2007

Keywords

  • Ara h 1 allergen
  • Equipment cleaning
  • Peanut proteins

ASJC Scopus subject areas

  • Biotechnology
  • Food Science

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