Development of a new method for improved identification and relative quantification of unknown metabolites in complex samples: Determination of a triterpenoid metabolic fingerprint for the in situ characterization of Ganoderma bioactive compounds

Jiri Adamec, Amber Jannasch, Shailesh Dudhgaonkar, Andrej Jedinak, Miroslav Sedlak, Daniel Sliva

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Ganoderma lucidum is a mushroom with a long history of medical applications. Research has demonstrated chemotherapeutic effects of G. lucidum in tissue culture, and bioactive fractions of the mushroom have been shown to contain high levels of triterpenoids and polysaccharides. In this study, we developed a new method for the detection of ganoderic acids and other triterpenes in Ganoderma mushroom extracts based on a post-biosynthetic stable isotope encoding technique. Overall, 57 doublets were identified as potential ganoderic acids and 11 of those matched with the database. Ganoderic acid A, F and H were confirmed by standards and their absolute concentrations were measured in GLT (GA A: 3.88 mg/g; GA F: 0.95 mg/g and GA H: 1.74 mg/g) and ReishiMax (GA A: 2.32 mg/g; GA F: 0.43 mg/g and GA H: 0.85 mg/g) extracts. The method was also used for the evaluation of bioavailability of triterpenes after an oral application and demonstrated the presence of G. lucidum triterpenes in plasma.

Original languageEnglish (US)
Pages (from-to)4052-4058
Number of pages7
JournalJournal of Separation Science
Volume32
Issue number23-24
DOIs
StatePublished - Dec 2009

Keywords

  • GSIST
  • Ganoderic acid
  • Isotope coding
  • Metabolomics
  • Profiling

ASJC Scopus subject areas

  • Analytical Chemistry
  • Filtration and Separation

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