Development of an affinity silica monolith containing α1-acid glycoprotein for chiral separations

Rangan Mallik, Hai Xuan, David S. Hage

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

An affinity monolith based on silica and containing immobilized α1-acid glycoprotein (AGP) was developed and evaluated in terms of its binding, efficiency and selectivity in chiral separations. The results were compared with data obtained for the same protein when used as a chiral stationary phase with HPLC-grade silica particles or monoliths based on a copolymer of glycidyl methacrylate (GMA) and ethylene dimethacrylate (EDMA). The surface coverage of AGP in the silica monolith was 18% higher than that obtained with silica particles and 61% higher than that measured for a GMA/EDMA monolith. The higher surface area of the silica monolith gave materials that contained 1.5- to 3.6-times more immobilized protein per unit volume when compared to silica particles or a GMA/EDMA monolith. The retention, efficiency and resolving power of the AGP silica monolith were evaluated by injecting two chiral analytes onto this column (i.e., R/S-warfarin and R/S-propranolol). In each case, the AGP silica monolith gave higher retention plus better resolution and efficiency than AGP columns containing silica particles or a GMA/EDMA monolith. The AGP silica monolith also gave lower back pressures and separation impedances than these other materials. It was concluded that silica monoliths can be valuable alternatives to silica particles or GMA/EDMA monoliths when used with AGP as a chiral stationary phase.

Original languageEnglish (US)
Pages (from-to)294-304
Number of pages11
JournalJournal of Chromatography A
Volume1149
Issue number2
DOIs
StatePublished - May 18 2007

Keywords

  • Affinity monolith
  • Chiral separation
  • Hydrazide method
  • Silica monolith
  • α-Acid glycoprotein

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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