TY - JOUR
T1 - Development of europium doped core-shell silica cobalt ferrite functionalized nanoparticles for magnetic resonance imaging
AU - Kevadiya, Bhavesh D.
AU - Bade, Aditya N.
AU - Woldstad, Christopher
AU - Edagwa, Benson J.
AU - McMillan, Jo Ellyn M.
AU - Sajja, Balasrinivasa R.
AU - Boska, Michael D.
AU - Gendelman, Howard E.
N1 - Funding Information:
The authors would like to thank Tom Bargar and Nicholas Conoan of the Electron Microscopy Core Facility (EMCF) at the UNMC for technical assistance. The EMCF is supported by state funds from the Nebraska Research Initiative (NRI) and the University of Nebraska Foundation, and institutionally by the Office of the Vice Chancellor for Research. The authors also would like to thank the UNMC Confocal Laser Scanning Microscopy and ICP-MS Core facilities and the Nebraska Center for Materials and Nanoscience at the University of Nebraska-Lincoln core facility for XRD, SQUID, AFM and HR-TEM. The authors would like to thank Melissa Mellon, Lirong Xu and Drs. Mariluz Arainga Ramirez, Prasanta Dash and Divya Prakash Gnanadhas for their thoughtful comments, active discussions and technical assistance. This work was supported in part by NIH Grant AG043540, DA028555, NS036126, NS034239, MH064570, NS043985, MH062261, AG043540 and DOD Grant 421-20-09A (HEG), the Carol Swarts Emerging Neuroscience Fund (HEG), a grant from the Nebraska Research Initiative (MB), and start-up funds from the Department of Pharmacology and Experimental Neuroscience (HEG).
Publisher Copyright:
© 2016 Acta Materialia Inc.
PY - 2017/2/1
Y1 - 2017/2/1
N2 - The size, shape and chemical composition of europium (Eu3+) cobalt ferrite (CFEu) nanoparticles were optimized for use as a “multimodal imaging nanoprobe” for combined fluorescence and magnetic resonance bioimaging. Doping Eu3+ ions into a CF structure imparts unique bioimaging and magnetic properties to the nanostructure that can be used for real-time screening of targeted nanoformulations for tissue biodistribution assessment. The CFEu nanoparticles (size ∼7.2 nm) were prepared by solvothermal techniques and encapsulated into poloxamer 407-coated mesoporous silica (Si-P407) to form superparamagnetic monodisperse Si-CFEu nanoparticles with a size of ∼140 nm. Folic acid (FA) nanoparticle decoration (FA-Si-CFEu, size ∼140 nm) facilitated monocyte-derived macrophage (MDM) targeting. FA-Si-CFEu MDM uptake and retention was higher than seen with Si-CFEu nanoparticles. The transverse relaxivity of both Si-CFEu and FA-Si-CFEu particles were r2 = 433.42 mM−1 s−1 and r2 = 419.52 mM−1 s−1 (in saline) and r2 = 736.57 mM−1 s−1 and r2 = 814.41 mM−1 s−1 (in MDM), respectively. The results were greater than a log order-of-magnitude than what was observed at replicate iron concentrations for ultrasmall superparamagnetic iron oxide (USPIO) particles (r2 = 31.15 mM−1 s−1 in saline) and paralleled data sets obtained for T2 magnetic resonance imaging. We now provide a developmental opportunity to employ these novel particles for theranostic drug distribution and efficacy evaluations. Statement of Significance A novel europium (Eu3+) doped cobalt ferrite (Si-CFEu) nanoparticle was produced for use as a bioimaging probe. Its notable multifunctional, fluorescence and imaging properties, allows rapid screening of future drug biodistribution. Decoration of the Si-CFEu particles with folic acid increased its sensitivity and specificity for magnetic resonance imaging over a more conventional ultrasmall superparamagnetic iron oxide particles. The future use of these particles in theranostic tests will serve as a platform for designing improved drug delivery strategies to combat inflammatory and infectious diseases.
AB - The size, shape and chemical composition of europium (Eu3+) cobalt ferrite (CFEu) nanoparticles were optimized for use as a “multimodal imaging nanoprobe” for combined fluorescence and magnetic resonance bioimaging. Doping Eu3+ ions into a CF structure imparts unique bioimaging and magnetic properties to the nanostructure that can be used for real-time screening of targeted nanoformulations for tissue biodistribution assessment. The CFEu nanoparticles (size ∼7.2 nm) were prepared by solvothermal techniques and encapsulated into poloxamer 407-coated mesoporous silica (Si-P407) to form superparamagnetic monodisperse Si-CFEu nanoparticles with a size of ∼140 nm. Folic acid (FA) nanoparticle decoration (FA-Si-CFEu, size ∼140 nm) facilitated monocyte-derived macrophage (MDM) targeting. FA-Si-CFEu MDM uptake and retention was higher than seen with Si-CFEu nanoparticles. The transverse relaxivity of both Si-CFEu and FA-Si-CFEu particles were r2 = 433.42 mM−1 s−1 and r2 = 419.52 mM−1 s−1 (in saline) and r2 = 736.57 mM−1 s−1 and r2 = 814.41 mM−1 s−1 (in MDM), respectively. The results were greater than a log order-of-magnitude than what was observed at replicate iron concentrations for ultrasmall superparamagnetic iron oxide (USPIO) particles (r2 = 31.15 mM−1 s−1 in saline) and paralleled data sets obtained for T2 magnetic resonance imaging. We now provide a developmental opportunity to employ these novel particles for theranostic drug distribution and efficacy evaluations. Statement of Significance A novel europium (Eu3+) doped cobalt ferrite (Si-CFEu) nanoparticle was produced for use as a bioimaging probe. Its notable multifunctional, fluorescence and imaging properties, allows rapid screening of future drug biodistribution. Decoration of the Si-CFEu particles with folic acid increased its sensitivity and specificity for magnetic resonance imaging over a more conventional ultrasmall superparamagnetic iron oxide particles. The future use of these particles in theranostic tests will serve as a platform for designing improved drug delivery strategies to combat inflammatory and infectious diseases.
KW - Biodistribution
KW - Cobalt ferrite
KW - Magnetic resonance imaging
KW - Monocyte-macrophages
KW - Multimodal imaging
KW - Nanoprobes
UR - http://www.scopus.com/inward/record.url?scp=85008208818&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85008208818&partnerID=8YFLogxK
U2 - 10.1016/j.actbio.2016.11.071
DO - 10.1016/j.actbio.2016.11.071
M3 - Article
C2 - 27916740
AN - SCOPUS:85008208818
VL - 49
SP - 507
EP - 520
JO - Acta Biomaterialia
JF - Acta Biomaterialia
SN - 1742-7061
ER -