Development of fluorescent contrast agents for optical imaging of mouse disease models

J. Kovar, M. Simpson, A. Schutz-Geschwender, X. Xu, W. M. Volcheck, E. Sevickmuraca, D. M. Olive

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Optical imaging is a rapidly developing field of research aimed at non-invasively interrogating animals for disease progression, determining the effects of a drug on a particular pathology, assessing the pharmacokinetic behavior of a drug, or identifying molecular biomarkers of disease. One of the key components of molecular imaging is the development of specific, targeted imaging contrast agents to assess mese biological processes. The development of robust fluorochrome-labeled optical agents is a process that is often underestimated in terms of its complexity. We describe here the development process and performance issues for three different optical agents: IRDye 800CW EGF (epidermal growth factor), IRDye® 800CW 2-DG (2-deoxy D-glucose), and an IRDye 680 BoneTag™. In vitro competitive assays were developed for two of the markers to demonstrate specificity. Specificity was confirmed in animal studies. Uptake of IRDye 800CW 2-DG was also examined by near-infrared confocal microscopy. Histological examinations were performed on target and non-target tissues following the completion of the imaging studies. The issues unique to the development of each labeled marker are discussed.

Original languageEnglish (US)
Title of host publicationMolecular Probes for Biomedical Applications II
DOIs
StatePublished - 2008
EventMolecular Probes for Biomedical Applications II - San Jose, CA, United States
Duration: Jan 21 2008Jan 22 2008

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume6867
ISSN (Print)1605-7422

Conference

ConferenceMolecular Probes for Biomedical Applications II
CountryUnited States
CitySan Jose, CA
Period1/21/081/22/08

Keywords

  • 2-deoxy-D-glucose
  • IRDye 680 epidermal growth factor
  • IRDye 800CW
  • Optical imaging

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Kovar, J., Simpson, M., Schutz-Geschwender, A., Xu, X., Volcheck, W. M., Sevickmuraca, E., & Olive, D. M. (2008). Development of fluorescent contrast agents for optical imaging of mouse disease models. In Molecular Probes for Biomedical Applications II [686707] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6867). https://doi.org/10.1117/12.760815