Development of murine embryos following electroporation

C. A. Schmotzer, M. E. Dunlap-Brown, S. P. Butler, W. H. Velander, F. C. Gwazdauskas

Research output: Contribution to journalArticle

Abstract

Purpose: The objective was to establish the parameters for reversible electroporation of murine embryos. Methods: In Trial 1, murine presumptive zygotes received an electrical pulse of 5, 10, or 20-μs duration, and one of five voltages (100, 200, 250, 300, or 400 V). In Trial 2, embryo orientation within the electroporation chamber was evaluated with 250 or 400 V at a pulse period of 10 μs. Results: Presumptive zygotes that received 400 V at each pulse length and zygotes exposed to 20 μs at each voltage had the lowest embryonic development (P < 0.05). Presumptive zygotes that received 250 V had higher development compared to 400 V, irrespective of orientation (P < 0.01), but development was lower than the controls (P < 0.01). Conclusions: Electrical stimulation of presumptive zygotes can have a detrimental impact on early embryo development, but low amounts of stimulation may allow for potential gene transfer in transgenic experimentation.

Original languageEnglish (US)
Pages (from-to)148-152
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Volume20
Issue number4
DOIs
StatePublished - Apr 1 2003

Keywords

  • Electroporation
  • Murine
  • Presumptive zygotes
  • Pulse period
  • Voltage

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Genetics(clinical)

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