Development of persistent ovarian follicles during synchronization of estrus influences the superovulatory response to FSH treatment in cattle

M. E. Wehrman; K. E. Fike; F. N. Kojima; E. G. Bergfeld; A. S. Cupp; V. Mariscal; T. Sanchez; I. E. Kinder

doi:10.1016/0093-691X(95)00406-X

Development of persistent ovarian follicles during synchronization of estrus influences the superovulatory response to FSH treatment in cattle

M. E. Wehrman, K. E. Fike, F. N. Kojima, E. G. Bergfeld, A. S. Cupp, V. Mariscal, T. Sanchez, I. E. Kinder

Animal Science

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

The synchronization of estrus with synthetic progestins or progesterone (P₄) results in the development of a large, persistent ovarian follicle. The objectives of the present study were to determine if development of a persistent ovarian follicle during synchronization of estrus suppresses recruitment of additional follicles during FSH treatment. On Day 5 of the estrous cycle (estrus=Day 0), beef cows were treated with 0.5 or 2.0 P₄ releasing intravaginal devices (PRIDs) for 8 d (Experiment 1, n=20), 5 or 2 d (Experiment 2, n=44) before initiation of FSH treatment. Prostaglandin F_2α (25 mg) was administered on Days 5 and 6. Superovulation was induced with 24 mg of recombinant bovine FSH (rbFSH, Experiment 1) or 28 mg of FSH-P (Experiment 2) over a 3- or 4-d period, respectively. The PRIDs were removed concurrently with the 5th injection of rbFSH or FSH-P. There was a treatment-by-day interaction (P<0.001) for the concentration of 17ß-estradiol in cows treated for 8, 5 or 2 d before FSH treatment. In Experiment 1, FSH treatment initiated 8 d after insertion of a 0.5 PRID did not affect the number of CL (6.9±1.4 vs 6.7±1.6), ova/embryos (3.7±1.3 vs 3.0±1.3) and transferable embryos (2.4±0.9 vs 3.0±0.9) compared with that of the 2.0 PRIDs. In Experiment 2, FSH treatment initiated 5 d after insertion of a 0.5 PRID decreased the number of CL (4.0±0.5 vs 8.3±0.8; P<0.001), ova/embryos (3.0±0.6 vs 5.9±1.2; P<0.03) and transferable embryos (2.3±0.6 vs 5.1±1.0; P<0.03) compared with that of a 2.0 PRID, respectively. Initiation of FSH treatment 2 d after insertion of a 0.5 PRID compared with a 2.0 PRID had no affect on the number of CL (8.0±2.1 vs 8.7±1.2), total ova (4.8±1.4 vs 6.9±1.4) and transferable embryos (2.9±1.2 vs 6.1±1.7). In conclusion, treatment with low doses of P₄ (0.5 PRID) for 5 d but not for 2 or 8 d before initiation of FSH treatment results in the development of a dominant ovarian follicle, which reduces recruitment of ovarian follicles, and the number of CL, total ova and transferable embryos.

Original language	English (US)
Pages (from-to)	593-610
Number of pages	18
Journal	Theriogenology
Volume	45
Issue number	3
DOIs	https://doi.org/10.1016/0093-691X(95)00406-X
State	Published - 1996

Keywords

Bovine
Ovarian follicular development
Progesterone
Superovulation

ASJC Scopus subject areas

Small Animals
Food Animals
Animal Science and Zoology
Equine

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10.1016/0093-691X(95)00406-X

Cite this

@article{a7b5a13806674366be0d57f27de6f6b3,

title = "Development of persistent ovarian follicles during synchronization of estrus influences the superovulatory response to FSH treatment in cattle",

abstract = "The synchronization of estrus with synthetic progestins or progesterone (P4) results in the development of a large, persistent ovarian follicle. The objectives of the present study were to determine if development of a persistent ovarian follicle during synchronization of estrus suppresses recruitment of additional follicles during FSH treatment. On Day 5 of the estrous cycle (estrus=Day 0), beef cows were treated with 0.5 or 2.0 P4 releasing intravaginal devices (PRIDs) for 8 d (Experiment 1, n=20), 5 or 2 d (Experiment 2, n=44) before initiation of FSH treatment. Prostaglandin F2α (25 mg) was administered on Days 5 and 6. Superovulation was induced with 24 mg of recombinant bovine FSH (rbFSH, Experiment 1) or 28 mg of FSH-P (Experiment 2) over a 3- or 4-d period, respectively. The PRIDs were removed concurrently with the 5th injection of rbFSH or FSH-P. There was a treatment-by-day interaction (P<0.001) for the concentration of 17{\ss}-estradiol in cows treated for 8, 5 or 2 d before FSH treatment. In Experiment 1, FSH treatment initiated 8 d after insertion of a 0.5 PRID did not affect the number of CL (6.9±1.4 vs 6.7±1.6), ova/embryos (3.7±1.3 vs 3.0±1.3) and transferable embryos (2.4±0.9 vs 3.0±0.9) compared with that of the 2.0 PRIDs. In Experiment 2, FSH treatment initiated 5 d after insertion of a 0.5 PRID decreased the number of CL (4.0±0.5 vs 8.3±0.8; P<0.001), ova/embryos (3.0±0.6 vs 5.9±1.2; P<0.03) and transferable embryos (2.3±0.6 vs 5.1±1.0; P<0.03) compared with that of a 2.0 PRID, respectively. Initiation of FSH treatment 2 d after insertion of a 0.5 PRID compared with a 2.0 PRID had no affect on the number of CL (8.0±2.1 vs 8.7±1.2), total ova (4.8±1.4 vs 6.9±1.4) and transferable embryos (2.9±1.2 vs 6.1±1.7). In conclusion, treatment with low doses of P4 (0.5 PRID) for 5 d but not for 2 or 8 d before initiation of FSH treatment results in the development of a dominant ovarian follicle, which reduces recruitment of ovarian follicles, and the number of CL, total ova and transferable embryos.",

keywords = "Bovine, Ovarian follicular development, Progesterone, Superovulation",

author = "Wehrman, {M. E.} and Fike, {K. E.} and Kojima, {F. N.} and Bergfeld, {E. G.} and Cupp, {A. S.} and V. Mariscal and T. Sanchez and Kinder, {I. E.}",

note = "Funding Information: Acknowledgments We thank Dr. M. Wilson of Granada Biosciences for the rbFSH, Dr. N. Mason for E, antisera, Dr. H. E. Grotjan, Jr. for the use of the Four Fit Program and The Upjohn Co. for providing prostaglandin F,,. This study is published as paper No. 11110, Journal Series Nebraska Agricultural Research Division. Research was supported by USDA CRGO 90-37240-5714. {\textquoteleft}Correspondence and reprint requests: James E. Kinder, A224j Animal Science, University of Nebraska, Lincoln, NE 68583-0908 USA; Fax:(402)472-6362.",

year = "1996",

doi = "10.1016/0093-691X(95)00406-X",

language = "English (US)",

volume = "45",

pages = "593--610",

journal = "Theriogenology",

issn = "0093-691X",

publisher = "Elsevier Inc.",

number = "3",

}

TY - JOUR

T1 - Development of persistent ovarian follicles during synchronization of estrus influences the superovulatory response to FSH treatment in cattle

AU - Wehrman, M. E.

AU - Fike, K. E.

AU - Kojima, F. N.

AU - Bergfeld, E. G.

AU - Cupp, A. S.

AU - Mariscal, V.

AU - Sanchez, T.

AU - Kinder, I. E.

N1 - Funding Information: Acknowledgments We thank Dr. M. Wilson of Granada Biosciences for the rbFSH, Dr. N. Mason for E, antisera, Dr. H. E. Grotjan, Jr. for the use of the Four Fit Program and The Upjohn Co. for providing prostaglandin F,,. This study is published as paper No. 11110, Journal Series Nebraska Agricultural Research Division. Research was supported by USDA CRGO 90-37240-5714. ‘Correspondence and reprint requests: James E. Kinder, A224j Animal Science, University of Nebraska, Lincoln, NE 68583-0908 USA; Fax:(402)472-6362.

PY - 1996

Y1 - 1996

N2 - The synchronization of estrus with synthetic progestins or progesterone (P4) results in the development of a large, persistent ovarian follicle. The objectives of the present study were to determine if development of a persistent ovarian follicle during synchronization of estrus suppresses recruitment of additional follicles during FSH treatment. On Day 5 of the estrous cycle (estrus=Day 0), beef cows were treated with 0.5 or 2.0 P4 releasing intravaginal devices (PRIDs) for 8 d (Experiment 1, n=20), 5 or 2 d (Experiment 2, n=44) before initiation of FSH treatment. Prostaglandin F2α (25 mg) was administered on Days 5 and 6. Superovulation was induced with 24 mg of recombinant bovine FSH (rbFSH, Experiment 1) or 28 mg of FSH-P (Experiment 2) over a 3- or 4-d period, respectively. The PRIDs were removed concurrently with the 5th injection of rbFSH or FSH-P. There was a treatment-by-day interaction (P<0.001) for the concentration of 17ß-estradiol in cows treated for 8, 5 or 2 d before FSH treatment. In Experiment 1, FSH treatment initiated 8 d after insertion of a 0.5 PRID did not affect the number of CL (6.9±1.4 vs 6.7±1.6), ova/embryos (3.7±1.3 vs 3.0±1.3) and transferable embryos (2.4±0.9 vs 3.0±0.9) compared with that of the 2.0 PRIDs. In Experiment 2, FSH treatment initiated 5 d after insertion of a 0.5 PRID decreased the number of CL (4.0±0.5 vs 8.3±0.8; P<0.001), ova/embryos (3.0±0.6 vs 5.9±1.2; P<0.03) and transferable embryos (2.3±0.6 vs 5.1±1.0; P<0.03) compared with that of a 2.0 PRID, respectively. Initiation of FSH treatment 2 d after insertion of a 0.5 PRID compared with a 2.0 PRID had no affect on the number of CL (8.0±2.1 vs 8.7±1.2), total ova (4.8±1.4 vs 6.9±1.4) and transferable embryos (2.9±1.2 vs 6.1±1.7). In conclusion, treatment with low doses of P4 (0.5 PRID) for 5 d but not for 2 or 8 d before initiation of FSH treatment results in the development of a dominant ovarian follicle, which reduces recruitment of ovarian follicles, and the number of CL, total ova and transferable embryos.

AB - The synchronization of estrus with synthetic progestins or progesterone (P4) results in the development of a large, persistent ovarian follicle. The objectives of the present study were to determine if development of a persistent ovarian follicle during synchronization of estrus suppresses recruitment of additional follicles during FSH treatment. On Day 5 of the estrous cycle (estrus=Day 0), beef cows were treated with 0.5 or 2.0 P4 releasing intravaginal devices (PRIDs) for 8 d (Experiment 1, n=20), 5 or 2 d (Experiment 2, n=44) before initiation of FSH treatment. Prostaglandin F2α (25 mg) was administered on Days 5 and 6. Superovulation was induced with 24 mg of recombinant bovine FSH (rbFSH, Experiment 1) or 28 mg of FSH-P (Experiment 2) over a 3- or 4-d period, respectively. The PRIDs were removed concurrently with the 5th injection of rbFSH or FSH-P. There was a treatment-by-day interaction (P<0.001) for the concentration of 17ß-estradiol in cows treated for 8, 5 or 2 d before FSH treatment. In Experiment 1, FSH treatment initiated 8 d after insertion of a 0.5 PRID did not affect the number of CL (6.9±1.4 vs 6.7±1.6), ova/embryos (3.7±1.3 vs 3.0±1.3) and transferable embryos (2.4±0.9 vs 3.0±0.9) compared with that of the 2.0 PRIDs. In Experiment 2, FSH treatment initiated 5 d after insertion of a 0.5 PRID decreased the number of CL (4.0±0.5 vs 8.3±0.8; P<0.001), ova/embryos (3.0±0.6 vs 5.9±1.2; P<0.03) and transferable embryos (2.3±0.6 vs 5.1±1.0; P<0.03) compared with that of a 2.0 PRID, respectively. Initiation of FSH treatment 2 d after insertion of a 0.5 PRID compared with a 2.0 PRID had no affect on the number of CL (8.0±2.1 vs 8.7±1.2), total ova (4.8±1.4 vs 6.9±1.4) and transferable embryos (2.9±1.2 vs 6.1±1.7). In conclusion, treatment with low doses of P4 (0.5 PRID) for 5 d but not for 2 or 8 d before initiation of FSH treatment results in the development of a dominant ovarian follicle, which reduces recruitment of ovarian follicles, and the number of CL, total ova and transferable embryos.

KW - Bovine

KW - Ovarian follicular development

KW - Progesterone

KW - Superovulation

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Development of persistent ovarian follicles during synchronization of estrus influences the superovulatory response to FSH treatment in cattle

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