TY - JOUR
T1 - Developmental and tissue-specific expression of the rod photoreceptor cGMP-gated ion channel gene
AU - Ahmad, Iqbal
AU - Redmond, Lori J.
AU - Barnstable, Colin J.
N1 - Funding Information:
We thank A. Butler for excellent technical assistance and R. Brown and H. Stopka for help with the manuscript. This work was supported by NIH grants EY00785 and EY05206, and by the Darien Lions and Research to Prevent Blindness Inc. IA is an ARVO/ALCON Postdoctoral Fellow and CJB is a Jules and Doris Stein Research to Prevent Blindness, Inc. Professor.
PY - 1990/11/30
Y1 - 1990/11/30
N2 - Probes against the retinal cGMP-gated cation channel were generated by PCR amplification of cDNA from rat and bovine retina. Southern and Northern analyses showed that the channel is encoded by a single gene that gives rise to a single mRNA species of 3.2 kb. Low levels of cGMP-gated channel RNA were detected in postnatal day 1 (PN 1) retinas and the amount increased to adult levels over the next two weeks of development. Screening of a number of tissues by Northern blot hybridization and by PCR amplification showed the channel to be expressed by heart and kidney as well as retina, but not by cerebellum, cerebral cortex, liver, muscle, olfactory bulb, spleen, testes or thymus.
AB - Probes against the retinal cGMP-gated cation channel were generated by PCR amplification of cDNA from rat and bovine retina. Southern and Northern analyses showed that the channel is encoded by a single gene that gives rise to a single mRNA species of 3.2 kb. Low levels of cGMP-gated channel RNA were detected in postnatal day 1 (PN 1) retinas and the amount increased to adult levels over the next two weeks of development. Screening of a number of tissues by Northern blot hybridization and by PCR amplification showed the channel to be expressed by heart and kidney as well as retina, but not by cerebellum, cerebral cortex, liver, muscle, olfactory bulb, spleen, testes or thymus.
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U2 - 10.1016/S0006-291X(05)81081-2
DO - 10.1016/S0006-291X(05)81081-2
M3 - Article
C2 - 2256934
AN - SCOPUS:0025694726
SN - 0006-291X
VL - 173
SP - 463
EP - 470
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -