Dexamethasone action inhibits the release of arachidonic acid from phosphatidylcholine during the suppression of yeast phagocytosis in macrophage cultures

Jeanne L. Becker, Robert J. Grasso, John S. Davis

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Dexamethasone suppresses phagocytosis of heat killed Saccharomyces cerevisiae in cultures of murine peritoneal macrophages. Recent observations suggest that dexamethasone induces a phagocytic inhibitory protein that suppresses yeast ingestion by inhibiting macrophage phospholipase A2 activity. The present investigation, therefore, examined whether macrophage lipid metabolism is modulated by dexamethasone. Control and steroid treated macrophages were allowed to incorporate radiolabeled arachidonate and were incubated subsequently in the absence and presence of yeast. Following ingestion by control macrophages, arachidonate from phosphatidylcholine was readily cleaved to free fatty acid and transferred to the neutral lipid fraction. In contrast, arachidonate release was inhibited in dexamethasone treated macrophages. These results suggest that the suppression of yeast phagocytosis by dexamethasone action may be associated with the inhibition of phospholipase A2 activity.

Original languageEnglish (US)
Pages (from-to)583-590
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume153
Issue number2
DOIs
StatePublished - Jun 16 1988

Keywords

  • AA
  • Arachidonic Acid
  • BPB
  • CH
  • Cholesterol
  • DAG
  • DEX
  • Dexamethasone
  • Diacylglycerol (diolein)
  • MAG
  • Monoacylglycerol (monopalmitoylglycerol)
  • PA
  • PC
  • PE
  • PI
  • PIP
  • PLA2
  • Phagocytosis Inhibitory Protein
  • Phosphatidic Acid
  • Phosphatidylcholine
  • Phosphatidylethanolamine
  • Phosphatidylinositol
  • Phospholipase A2
  • TAG
  • TLC
  • Thin Layer Chromatography
  • Triacylglycerol (triolein)
  • p-Bromophenacyl Bromide

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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