Dexamethasone inhibits α-fetoprotein gene expression in developing mouse liver

Patricia Commer; Charles Schwartz; Steven Tracy; Taiki Tamaoki; Jen Fu Chiu

doi:10.1016/0006-291X(79)92149-1

Dexamethasone inhibits α-fetoprotein gene expression in developing mouse liver

Patricia Commer, Charles Schwartz, Steven Tracy, Taiki Tamaoki, Jen Fu Chiu

Research output: Contribution to journal › Article › peer-review

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Abstract

The effect of dexamethasone on α-fetoprotein gene expression was studied in newborn mice. The treatment of 3 day old mice with 1 μg/gm body weight of dexamethasone twice a day reduced the α-fetoprotein level in serum to about 2.5% of the normal control level after 4 days of administration. The level of α-fetoprotein mRNA sequences in the cytoplasm decreased to about 1% of the control over this same period. Furthermore, only an extremely low concentration of α-fetoprotein mRNA sequences in nuclear RNA could be detected even at a high C_rot. These results suggest that dexamethasone inhibits transcription of the α-fetoprotein gene in newborn mice.

Original language	English (US)
Pages (from-to)	1294-1299
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	89
Issue number	4
DOIs	https://doi.org/10.1016/0006-291X(79)92149-1
State	Published - Aug 28 1979
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/0006-291X(79)92149-1

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@article{2b2292feff7f45249af9007b7511dee3,

title = "Dexamethasone inhibits α-fetoprotein gene expression in developing mouse liver",

abstract = "The effect of dexamethasone on α-fetoprotein gene expression was studied in newborn mice. The treatment of 3 day old mice with 1 μg/gm body weight of dexamethasone twice a day reduced the α-fetoprotein level in serum to about 2.5% of the normal control level after 4 days of administration. The level of α-fetoprotein mRNA sequences in the cytoplasm decreased to about 1% of the control over this same period. Furthermore, only an extremely low concentration of α-fetoprotein mRNA sequences in nuclear RNA could be detected even at a high Crot. These results suggest that dexamethasone inhibits transcription of the α-fetoprotein gene in newborn mice.",

author = "Patricia Commer and Charles Schwartz and Steven Tracy and Taiki Tamaoki and Chiu, {Jen Fu}",

note = "Funding Information: This work was supported by USPHS grant No. ROl CA25098, awarded by the National Cancer Institute to Dr. Chiu. It was also supported in part by the Medical Research Council in Canada, the National Cancer Institute of Canada, and the Alberta Heritage Trust Fund, awarded to Dr. Tamaoki. Dr. Schwartz is postdoctoral trainee of Cancer Biology Training grant USPHS grant No. T32 CA09286. Dr. Tamaoki is Research Associate of the National Cancer Institute of Canada. The authors are indebted to Dr. Joseph W. Beard at the Life Sciences, Inc., St. Petersberg, Florida for kindly providing us with avian myeloblastosis virus reverse transcriptase.",

year = "1979",

month = aug,

day = "28",

doi = "10.1016/0006-291X(79)92149-1",

language = "English (US)",

volume = "89",

pages = "1294--1299",

journal = "Biochemical and Biophysical Research Communications",

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publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Dexamethasone inhibits α-fetoprotein gene expression in developing mouse liver

AU - Commer, Patricia

AU - Schwartz, Charles

AU - Tracy, Steven

AU - Tamaoki, Taiki

AU - Chiu, Jen Fu

N1 - Funding Information: This work was supported by USPHS grant No. ROl CA25098, awarded by the National Cancer Institute to Dr. Chiu. It was also supported in part by the Medical Research Council in Canada, the National Cancer Institute of Canada, and the Alberta Heritage Trust Fund, awarded to Dr. Tamaoki. Dr. Schwartz is postdoctoral trainee of Cancer Biology Training grant USPHS grant No. T32 CA09286. Dr. Tamaoki is Research Associate of the National Cancer Institute of Canada. The authors are indebted to Dr. Joseph W. Beard at the Life Sciences, Inc., St. Petersberg, Florida for kindly providing us with avian myeloblastosis virus reverse transcriptase.

PY - 1979/8/28

Y1 - 1979/8/28

N2 - The effect of dexamethasone on α-fetoprotein gene expression was studied in newborn mice. The treatment of 3 day old mice with 1 μg/gm body weight of dexamethasone twice a day reduced the α-fetoprotein level in serum to about 2.5% of the normal control level after 4 days of administration. The level of α-fetoprotein mRNA sequences in the cytoplasm decreased to about 1% of the control over this same period. Furthermore, only an extremely low concentration of α-fetoprotein mRNA sequences in nuclear RNA could be detected even at a high Crot. These results suggest that dexamethasone inhibits transcription of the α-fetoprotein gene in newborn mice.

AB - The effect of dexamethasone on α-fetoprotein gene expression was studied in newborn mice. The treatment of 3 day old mice with 1 μg/gm body weight of dexamethasone twice a day reduced the α-fetoprotein level in serum to about 2.5% of the normal control level after 4 days of administration. The level of α-fetoprotein mRNA sequences in the cytoplasm decreased to about 1% of the control over this same period. Furthermore, only an extremely low concentration of α-fetoprotein mRNA sequences in nuclear RNA could be detected even at a high Crot. These results suggest that dexamethasone inhibits transcription of the α-fetoprotein gene in newborn mice.

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IS - 4

ER -

Dexamethasone inhibits α-fetoprotein gene expression in developing mouse liver

Abstract

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