The paired-end sequencing approach provides a powerful means for studying genome structural changes. We developed the DGS (ditag genome scanning) technique that collects the paired-end tags from restriction fragments. We established the detailed protocols for collecting the paired-end tag sequences using different next-generation sequencing platforms and developed an informatics pipeline for DGS data mapping analysis. Our applications of DGS in multiple genome analyses show that DGS provides a tool for genome structural studies with high specificity and versatile resolution.
- DGS (ditag genome scanning)
- Genome structural analysis
- Restriction-based paired-end sequencing
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)