DGS (Ditag Genome Scanning) - A Restriction-Based Paired-End Sequencing Approach for Genome Structural Analysis

The paired-end sequencing approach provides a powerful means for studying genome structural changes. We developed the DGS (ditag genome scanning) technique that collects the paired-end tags from restriction fragments. We established the detailed protocols for collecting the paired-end tag sequences using different next-generation sequencing platforms and developed an informatics pipeline for DGS data mapping analysis. Our applications of DGS in multiple genome analyses show that DGS provides a tool for genome structural studies with high specificity and versatile resolution.