DGS (Ditag Genome Scanning) - A Restriction-Based Paired-End Sequencing Approach for Genome Structural Analysis

Jun Chen, Yeong C. Kim, San Ming Wang

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The paired-end sequencing approach provides a powerful means for studying genome structural changes. We developed the DGS (ditag genome scanning) technique that collects the paired-end tags from restriction fragments. We established the detailed protocols for collecting the paired-end tag sequences using different next-generation sequencing platforms and developed an informatics pipeline for DGS data mapping analysis. Our applications of DGS in multiple genome analyses show that DGS provides a tool for genome structural studies with high specificity and versatile resolution.

Original languageEnglish (US)
Title of host publicationTag-Based Next Generation Sequencing
PublisherWiley-VCH
Pages277-285
Number of pages9
ISBN (Print)9783527328192
DOIs
StatePublished - Jan 23 2012

Keywords

  • DGS (ditag genome scanning)
  • Genome structural analysis
  • Methods
  • Restriction-based paired-end sequencing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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